NSF Young Investigator

体外选择技术将用于确定含有富含精氨酸基序的蛋白质如何特异性识别其同源rna。将扫描界面的RNA和蛋白质伴侣可用的可能序列基序，并尝试识别蛋白质的“代码”：确定核酸相互作用。由蛋白质和核酸形成的互补界面中的共性对于理解大多数生命过程至关重要。在体外将进行遗传选择，以确定哪一组蛋白质序列基序。分子识别“问题”的可能“答案”的范围将被调查和改进，如果可能的话。＊＊＊

9458176 Ellington In vitro selection techniques will be used to determine how proteins containing arginine-rich motifs can specifically recognize their cognate RNAs. The possible sequence motifs available to both RNA and protein partners of the interface will be scanned and an attempt to identify `codes' for protein: nucleic acid interactions determined. %%% Commonalities in the complementary interfaces formed by proteins and nucleic acids are critical to understanding most life processes. In vitro genetic selections will be done in order to determine what set of protein sequence motifs. The range of possible `answers' to the molecular recognition `problems' will be investigated and improvements made, if possible. ***