Gold and Combined Fluorescent/Gold Labels for Solid-Phase DNA Synthesis
Gold and Combined Fluorescent/Gold Labels for Solid-Phase DNA Synthesis
批准号:
9460234
负责人:
Richard Powell
金额:
$6.5万
依托单位:
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1995
资助国家:
美国
项目状态:
已结题
起止时间:
1995-01-01 至 1995-09-30
中文摘要
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英文摘要
Novel phosphoramidite reagents are proposed which will be used to incorporate gold cluster complexes and combined fluorescent/gold labels into oligonucleotides during automated solid state synthesis. The labels will be synthetically modified to withstand the synthesis and deprotection conditions. Gold and fluorescent/gold conjugated phosphoramidites will be prepared in forms specific for labeling at the 5' end of the oligonucleotide, at the 3' end via derivatization of a controlled pore glass support, and at any intermediate position; the synthesis of an adenosine phosphoramidite labeled in the 2' position is also proposed. The labels will be incorporated into oligonucleotide probes which will be tested in hybridization studies on blots, and in situ electron microscopy hybridization studies. The sensitivity, penetration and resolution of the labeled nucleotides will be evaluated relative to analogous biotinylated probes detected by a secondary probe bearing the same label. It is anticipated that synthetic probes which incorporate the new labels will achieve unprecedented resolution in the electron microscope, and blot sensitivities equal to or better than current radioisotopic or chemiluminescent techniques.
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Knowledges, resources and legal regimes: the new geopolitics of the Polar Regions
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批准号:ES/H000763/1
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项目类别:Research Grant
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资助金额:$0.48万
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财政年份:2010
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负责人:Richard Powell
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依托单位:
Knowledges, resources and legal regimes: the new geopolitics of the Polar Regions
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批准号:RES-451-26-0661-A
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项目类别:Research Grant
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资助金额:$0.0万
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财政年份:2010
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负责人:Richard Powell
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依托单位:
The sociopolitical, environmental and technological implications of climatic changes in the Circumpolar Arctic for UK En
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批准号:ES/E017223/1
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项目类别:Fellowship
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资助金额:$34.27万
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财政年份:2007
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负责人:Richard Powell
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依托单位:
SBIR Phase I: Autometallographic Fabrication of Copper Interconnects
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批准号:9860786
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项目类别:Standard Grant
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资助金额:$10.0万
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财政年份:1999
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负责人:Richard Powell
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依托单位:
SBIR PHASE I: Metal Cluster and Combined Flourescent/Gold Labels for Peptide Synthesis
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批准号:9561345
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项目类别:Standard Grant
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资助金额:$7.5万
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财政年份:1996
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负责人:Richard Powell
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依托单位:
Molecular Cytoskeletal Microscopy Probes and Biological Wires
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批准号:9360219
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项目类别:Standard Grant
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资助金额:$6.5万
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财政年份:1994
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负责人:Richard Powell
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依托单位:
Laser Spectroscopy Investigations of Dynamical Processes of Ions in Solids
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批准号:8722350
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项目类别:Continuing Grant
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资助金额:$12.92万
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财政年份:1988
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负责人:Richard Powell
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依托单位:
International Laser Science Conference; Seattle, Washington;October 19-24, 1986 (Physics)
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批准号:8609149
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项目类别:Standard Grant
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资助金额:$0.5万
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财政年份:1986
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负责人:Richard Powell
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依托单位:
Laser Spectroscopy Techniques for Investigating Radiationless Processes of Ions in Solids (Materials Research)
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批准号:8216551
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项目类别:Continuing Grant
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资助金额:$12.75万
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财政年份:1983
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负责人:Richard Powell
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依托单位:
Energy Transfer in Europium-Doped, Alkali-Halide Single Crystals
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批准号:8120975
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项目类别:Standard Grant
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资助金额:$0.53万
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财政年份:1982
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负责人:Richard Powell
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依托单位:
Use of Laser Spectroscopy Techniques For Investigating Energy Transfer Among Ions in Crystals
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批准号:7916152
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项目类别:Continuing Grant
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资助金额:$8.15万
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财政年份:1980
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负责人:Richard Powell
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依托单位:
Equipment For the Expansion of the Laser Facility
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批准号:7707453
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项目类别:Standard Grant
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资助金额:$1.22万
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财政年份:1977
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负责人:Richard Powell
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依托单位:
海外基金