New Fusion Protein Systems Designed to Avoid Inclusion Body Formation and Improve Protein Purification

CTS-9502235俄克拉何马大学的Roger Harrison研究将通过避免这些蛋白作为不溶性包涵体的初始表达来改进重组蛋白的纯化。一个主要的目标是测试新的融合蛋白，预计将提高通常不溶性重组蛋白的溶解度。在大肠杆菌中具有高溶解度的四种蛋白质将与已知溶解度低的两种不同的靶蛋白结合。纯化表达水平最高的融合蛋白。从融合蛋白中分离纯化目标蛋白并进一步鉴定。该实验将测试一个预测融合蛋白溶解度的模型的有效性。该结果可能导致融合蛋白技术的根本性进展，这可能对生物技术产生重大影响。

CTS-9502235 Roger Harrison U of Oklahoma Research will be performed to improve the purification of recombinant proteins by avoiding the initial expression of these proteins as insoluble inclusion bodies. A primary goal is to test new fusion proteins that are predicted to enhance the solubility of normally insoluble recombinant proteins. Four proteins with very high solubility in Escherichia coli will be combined with two different target proteins known to have low solubility. The fusion proteins with highest expression levels will be purified. The target protein will be purified after its cleavage from the fusion protein and characterized further. The experiments will test the validity of a model proposed to predict the solubility of fusion proteins. The results may lead to fundamental advances in fusion protein technology which could have significant impact in biotechnology.