Molecular Analysis of the Brome Mosaic Virus RNA-dependent RNA Polymerase

雀麦花叶病毒 RNA 依赖性 RNA 聚合酶的分子分析

• 批准号: 9507344
• 负责人: C. Cheng Kao
• 金额: $ 25.9万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9507344&HistoricalAwards=false
• 关键词: Molecular; Analysis; Brome; Mosaic; Virus

9507344 Kao Viruses with genomes of (+)-stranded RNAs make up a diverse group that include economically important pathogens of animals and plants. The mechanism of replication of these RNA genomes is not understood well, and what roles host proteins play are just beginning to be determined. The overall goal of this research is to understand the mechanism by which the model (+)-strand RNA virus, brome mosaic virus (BMV), replicates its genome. Experiments will focus on two key areas, the identification and characterization of host factors involved in viral RNA replication, and the interaction of viral proteins within the RNA-dependent RNA polymerase. The anticipated results are important because they will elucidate fundamental aspects of viral RNA replication, including the role of host proteins and the interaction of viral proteins. Many, if not all, of these mechanistic details should prove to be common among viral pathogens with (+)-strand RNA genomes. Advances in our understanding of RNA replication will provide the basis for the development of new strategies for the inhibition of viral infection. %%% Viruses with genomes of (+)-stranded RNAs make up a diverse group that include economically important pathogens of animals and plants. The mechanism of replication of these RNA genomes is not understood well, and what roles host proteins play are just beginning to be determined. The overall goal of this research is to understand the mechanism by which the model (+)-strand RNA virus, brome mosaic virus (BMV), replicates its genome. The anticipated results are important because they will elucidate fundamental aspects of viral RNA replication, including the role of host proteins and the interaction of viral proteins. Many, if not all, of these mechanistic details should prove to be common among viral pathogens with (+)-strand RNA genomes. Advances in our understanding of RNA replication will provide the basis for the development of new strategies for the inhibition of viral infection. ***

9507344具有（+）-链RNA基因组的Kao病毒组成了包括动物和植物的经济上重要的病原体的多样化的组。这些RNA基因组的复制机制还不清楚，宿主蛋白质所起的作用也刚刚开始确定。本研究的总体目标是了解模式（+）链RNA病毒雀麦花叶病毒（BMV）复制其基因组的机制。实验将集中在两个关键领域，参与病毒RNA复制的宿主因子的鉴定和表征，以及RNA依赖的RNA聚合酶内病毒蛋白的相互作用。预期的结果是重要的，因为它们将阐明病毒RNA复制的基本方面，包括宿主蛋白质的作用和病毒蛋白质的相互作用。许多（如果不是全部）这些机制的细节应该证明是常见的病毒病原体（+）链RNA基因组。我们对RNA复制理解的进展将为开发抑制病毒感染的新策略提供基础。 具有（+）-链RNA基因组的病毒组成了一个多样化的组，包括动物和植物的经济上重要的病原体。这些RNA基因组的复制机制还不清楚，宿主蛋白质所起的作用也刚刚开始确定。本研究的总体目标是了解模式（+）链RNA病毒雀麦花叶病毒（BMV）复制其基因组的机制。预期的结果是重要的，因为它们将阐明病毒RNA复制的基本方面，包括宿主蛋白质的作用和病毒蛋白质的相互作用。许多（如果不是全部）这些机制的细节应该证明是常见的病毒病原体（+）链RNA基因组。我们对RNA复制理解的进展将为开发抑制病毒感染的新策略提供基础。 ***