Development of a High Throughput Genetic Analysis Laboratory

从人类基因组计划和其他模式系统衍生的技术为研究许多重要的生物学问题以及动植物发病机制提供了一种极其有效和经济的方法。此外，提供现代技术方面的必要设备和培训显然是明尼苏达大学在履行其赠地使命方面的一项重要作用。最近和未来的教师招聘在明尼苏达大学圣保罗校区有必要发展一个核心的高通量基因分析实验室。此外，包括农学院、生物科学学院、医学院和兽医学院在内的大学战略规划使明尼苏达大学认识到，现在有机会在圣保罗校区建立一个国际认可的中心，重点研究新兴的DNA技术。最重要的是，正如个别项目描述所示，已经存在大量研究人员，他们对高通量DNA测序和基因分型能力有需求。这些技术将允许利用核和线粒体DNA基因的DNA测序来解决有关物种形成模式的问题，植物、细菌、原生动物、鸟类和哺乳动物之间的系统发育关系。该实验室开发的技术将为监测环境变化、鉴定新出现的植物和动物病原体以及针对特定生理特征的直接基因型选择的策略提供见解。与这些技术相关的知识环境将促进大学之间以及与私营部门之间的新伙伴关系。最后，这个基础设施将有助于加强本科生、研究生和专业学生的招聘，同时为现有的本科生和毕业生提供新的培训机会。这一战略冒险的关键是合适的教师的存在，他们将刺激新的跨学科研究项目，并将带来与这些新兴技术相关的新技能。目前，在圣保罗校区，食品动物生物技术中心包括几个不同的研究项目，其目标是通过识别和调节影响生长、发育、繁殖性能、饲料转化效率、泌乳和抗病性的基因，开发与动物生产相关的新分子技术。圣保罗校区植物分子遗传学研究所的目标是跨越分子，细胞和整个植物水平，并补充大学的植物育种计划。最后，明尼苏达大学生物科学学院的教师在遗传学和发育生物学方面发挥着领导作用。为了高效和有效地利用这个多研究员实验室，还将提供技术支持，作为机构匹配的一部分。为了补充这些现有的项目，兽医学院致力于招聘新的教师，他们将带来与这些需要广泛仪器的新兴技术相关的新技能。这些人将有已经建立的记录，将有助于刺激未来的申请和进一步的校际互动。本提案的几位参与者将在这些搜寻委员会任职。此外，为了刺激教师招聘，这个核心实验室还将在大学内外提供训练有素的支持人员，以便在整个地区使用现代设备。

Technologies derived from the genome projects for humans and other model systems offer an extremely effective and economical approach for studying many important biological questions as well as mechanisms of animal and plant pathogenesis. Furthermore, the availability of the necessary equipment and training in modern technologies is clearly an important role of the University of Minnesota in serving its Land Grant Mission. Recent and future faculty recruitment on the University of Minnesota, St. Paul Campus has necessitated the development of a core High Throughput Genetic Analysis Laboratory. In addition, university strategic planning including the Colleges of Agriculture, Biological Sciences, Medicine, and Veterinary Medicine lead to the recognition that the University of Minnesota now has the opportunity to establish an internationally recognized center focusing on emerging DNA technologies on the St. Paul Campus. Most importantly, as illustrated by the individual project descriptions, is the already present critical mass of researchers who have existing needs for high throughput DNA sequencing and genotyping capabilities. Such technologies will permit the utilization of DNA sequencing of nuclear and mitochondrial DNA genes to address questions concerning speciation patterns, phylogenetic relationships among plants, bacteria, protozoan, birds and mammals. Technologies developed by the laboratory will provide insights into strategies for monitoring environmental changes, the identification of emerging plant and animal pathogens, and direct genotypic selection for specific physiological characteristics. The intellectual environment associated with such technologies will foster new partnerships both between colleges as well as with the private sector. Finally, this infrastructure will serve to enhance undergraduate, graduate and professional student recruitment while providing new training opportunities for current undergraduate and graduates. Critical for this strategic venture is the presence of appropriate faculty who will stimulate new interdisciplinary research programs and who will bring new skills associated with these emerging technologies. At present on the St. Paul Campus, the Food Animal Biotechnology Center encompasses several different research programs that have as their goal to develop new molecular technologies associated with animal production through the identification and modulation of genes affecting growth, development, reproductive performance, efficiency of feed conversion, lactation and disease resistance. The goal of the Plant Molecular Genetics Institute on the St. Paul Campus is to span the molecular, cellular, and whole plant levels and to complement the University's plant breeding programs. Finally, faculty in the College of Biological Sciences play a leadership role in genetics and developmental biology at the University of Minnesota. In order to provide for efficient and effective utilization of this multiinvestigator laboratory, technical support will also be provided as part of the institutional match. To complement these existing programs, the College of Veterinary Medicine is committed to hiring new faculty who will bring new skills associated with these emerging technologies that require extensive instrumentation. These individuals, who will have an already established track record, will serve to stimulate future applications and further intercollegiate interactions. Several of the participants of this proposal will serve on theses search committees. In addition, to stimulating faculty recruitment, this core laboratory will also provide trained support staff, within and outside the university, to permit contemporary usage of the equipment throughout the region.