An Automated 15N, 13C Analyzer for the University of Texas Marine Science Institute, Port Aransas, Texas

德克萨斯州阿兰瑟斯港德克萨斯大学海洋科学研究所的自动 15N、13C 分析仪

• 批准号: 9512847
• 负责人: Ellery Ingall
• 金额: $ 10.3万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-01-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9512847&HistoricalAwards=false
• 关键词: Automated; 15N; 13C; Analyzer; University

Purchase of an automated 15N, 13C analyzer for the University of Texas Marine Science Institute to replace a 25 year old isotope ratio mass spectrometer and associated high vacuum sample preparation lines is proposed. Although tens of thousands of samples have been analyzed using the present system, it is currently inoperable and requires a major overhaul. However, because of the system's age, its relatively primitive technology, and demand by several users, any repairs to the instrument are unlikely to be cost effective over the long term or meet the expectations of the scientific research staff at the Institute. Instead, the purchase of a new analyzer represents a substantial advance in technological capabilities which will open additional avenues of research for the scientists at the Institute as well as providing isotopic data for currently funded research programs. The proposed and currently funded research avenues include microbiological, ecological, physiological and chemical studies. This research will use the stable isotope compositions of bioactive elements, such as C and N, to delineate metabolic pathways, understand food webs in natural environments, and determine the sources, transformations and fates of these elements in ecosystems. The proposed analyzer is a compact instrument which determines the carbon and nitrogen isotopic ratios of gases, solids and liquids in their natural state with minimal sample preparation. Expensive and time consuming sample preparation on high vacuum lines with subsequent transfer of purified sample gases to a mass spectrometer is avoided in the proposed system since sample processing and isotopic analysis are integrated into one unit. An additional advantage of the proposed system over those presently available to Institute scientists is that samples with natural abundance of the 13C and 15N isotopes as well as isotopically amended samples used in tracer studies can be analyzed on one instrument.

提出了为取代25岁的同位素比率质谱仪和相关的高真空样品制备线的自动化15N，13C分析仪。尽管已经使用本系统分析了数以万计的样本，但目前无法操作，需要进行重大大修。但是，由于系统的年龄，其相对原始的技术以及几个用户的需求，对该仪器的任何维修都不太可能长期具有成本效益，或者满足研究所科学研究人员的期望。 取而代之的是，购买新分析仪代表了技术能力的重大进步，这将为研究所的科学家开放其他研究途径，并为当前资助的研究计划提供同位素数据。拟议的和目前资助的研究途径包括微生物，生态，生理和化学研究。这项研究将使用生物活性元素（例如C和N）的稳定同位素组成来描述代谢途径，了解自然环境中的食物网，并确定生态系统中这些元素的来源，转化和命运。提出的分析仪是一种紧凑型仪器，它确定了其自然状态的气体，固体和液体的碳和氮同位素比，样品制备最少。在拟议的系统中避免了昂贵且耗时的样品制备，随后将纯化的样品气体转移到质谱仪中，因为样品处理和同位素分析被整合到一个单元中。该系统的另一个优点比目前可用于提出科学家的系统的另一个优点是，可以在一种仪器上分析具有13C和15N同位素的自然丰度以及用于示踪剂研究中使用的同位素修订的样品的样品。