Cryo unit and liquid helium cooled inverse 1H-13C-15N tripel-resonance cryoprobe

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We focus our research - especially within the CRC 1093 - on huge protein machines, their interaction with supramolecular ligands as well as on the interaction of these ligands with protein-complexes and platform proteins. All these complexes consist of hundreds of amino acids and their molecular weights often reach several hundreds of kilodaltons or even megadaltons. An often observed drawback of these systems are their poor solubilities. When performing NMR spectroscopy with such systems it becomes necessary to push the limitations of the spectrometers and demand highest sensitivity from the setup. Even for a 700 MHz machine most of the experiments recorded for our research need a cryo unit and a cryo probe to enhance sensitivity and signal strength to a factor where useful spectra can be obtained. According to an end-of-service call of the producer, our existing cryo unit is not longer maintained by the respective company. In addition, our probe head, which is running since 2006 becomes more and more susceptible to failure. To serve our needs and to continue and proceed our research within the CRC and on other DFG funded projects, we have to replace the cryo unit and the cryo probe as soon as possible.