Cryo unit and liquid helium cooled inverse 1H-13C-15N tripel-resonance cryoprobe

冷冻装置和液氦冷却逆 1H-13C-15N 三重共振冷冻探头

• 批准号: 445525856
• 金额: --
• 依托单位: Abteilung Strukturelle und Medizinische Biochemie
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/445525856?language=en
• 关键词: Cryo; unit; liquid; helium; cooled

We focus our research - especially within the CRC 1093 - on huge protein machines, their interaction with supramolecular ligands as well as on the interaction of these ligands with protein-complexes and platform proteins. All these complexes consist of hundreds of amino acids and their molecular weights often reach several hundreds of kilodaltons or even megadaltons. An often observed drawback of these systems are their poor solubilities. When performing NMR spectroscopy with such systems it becomes necessary to push the limitations of the spectrometers and demand highest sensitivity from the setup. Even for a 700 MHz machine most of the experiments recorded for our research need a cryo unit and a cryo probe to enhance sensitivity and signal strength to a factor where useful spectra can be obtained. According to an end-of-service call of the producer, our existing cryo unit is not longer maintained by the respective company. In addition, our probe head, which is running since 2006 becomes more and more susceptible to failure. To serve our needs and to continue and proceed our research within the CRC and on other DFG funded projects, we have to replace the cryo unit and the cryo probe as soon as possible.

我们的研究重点是巨大的蛋白质机器，它们与超分子配体的相互作用，以及这些配体与蛋白质复合物和平台蛋白的相互作用，特别是在CRC 1093中。所有这些复合物都由数百个氨基酸组成，它们的分子量往往达到数百千道尔顿甚至兆道尔顿。这些系统的一个经常观察到的缺点是它们的可解析度很差。当使用这种系统进行核磁共振波谱分析时，有必要突破光谱仪的限制，并要求设置具有最高的灵敏度。即使对于700mhz的机器，为我们的研究记录的大多数实验也需要一个低温装置和一个低温探头，以提高灵敏度和信号强度，从而获得有用的光谱。根据生产商的服务终止电话，我们现有的冷冻装置不再由各自的公司维护。此外，我们从2006年开始运行的探头越来越容易出现故障。为了满足我们的需要，并继续进行我们在CRC和其他DFG资助项目内的研究，我们必须尽快更换低温装置和低温探针。