Analytical Chemical Analysis in an Ecological Context: a High Pressure Liquid Chromatography Workstation, Fluorometer and Spectrometer

提出了研究植物与非生物环境相互作用的机制和生态方面的计划；微生物，包括菌根；还有食草昆虫。我们要求资助一个高压液相色谱（HPLC）工作站(贝克曼仪器生物分离梯度HPLC“系统金”（型号126可编程溶剂模块和型号166紫外/可见光检测器）和一个生物rad自动采样系统（型号AS-100），一个荧光计Perkin Elmer型号LS 50B“发光光谱仪”，和一个日立U-1100分光光度计。这些系统将用于支持科罗拉多大学三个实验室的研究；所有这些都涉及对生态过程中重要的各种化合物的分析和定量。在迪恩·鲍尔斯教授的实验室里，研究的重点是分析在营养相互作用（植物-食草昆虫-天敌）中重要的植物和昆虫的一级和二级化合物。Steve Schrnidt教授实验室的研究主要集中在两个领域。首先，研究了微生物对五氯酚（PCP）的生物降解；第二个是和Russell Monson教授合作的项目。这项合作研究在几个层面上研究高山养分循环：高山植物吸收氨基酸的过程和机制，以及与植物-微生物（包括菌根）相互作用有关的氨基酸吸收。这三个实验室的努力将大大加强所要求的设备的可用性，这些设备目前是不可用的，不可靠的和过时的，或大量用于其他实验室的研究。

A plan is presented to study the mechanistic and ecological aspects of plant interactions with the abiotic environment; with microbes, including mycorrhizae; and with herbivorous insects. We request funds for a High Pressure Liquid Chromatography (HPLC) Workstation (Beckman Instrument Bioseparation Gradient HPLC "System Gold" (Model 126 Programmable Solvent Module and Model 166 Ultraviolet/Visible Detector) and a Bio-Rad Automatic Sampling System (Model AS-100) , a fluorometer Perkin Elmer model LS 50B "Luminescence Spectrometer" , and a spectrophotometer Hitachi U-1100 Spectrophotometer with sample sipper . These systems will be used to support research in three laboratories at the University of Colorado; all of which are involved in the analysis and quantification of a variety of chemical compounds important in ecological processes. In the laboratory of Professor Deane Bowers, research is focused on the analysis of plant and insect primary and secondary compounds that are important in tritrophic interactions (plants-herbivorous insects-natural enemies). Research in the laboratory of Professor Steve Schrnidt is in two major areas. In the first, biodegradation of pentachlorophenol (PCP) by microorganisms is studied; and the second is a collaborative project with Professor Russell Monson. This collaborative research studies alpine nutrient cycling at several levels: the processes and mechanisms of amino acid uptake by alpine plants, and amino acid uptake in relation to plant-microbe interactions, including mycorrhizae. The efforts of these three laboratories will be greatly enhanced by the availability ofthe requested equipment, which is presently unavailable, unreliable and out-of-date, or heavily used for research in other laboratories.