Analytical Chemical Analysis in an Ecological Context: a High Pressure Liquid Chromatography Workstation, Fluorometer and Spectrometer

提出了一个计划，以研究植物与非生物环境的相互作用的机制和生态方面，与微生物，包括真菌和草食性昆虫。我们申请资金用于高压液相色谱（HPLC）工作站（贝克曼仪器生物分离梯度HPLC“System Gold”（126型可编程溶剂模块和166型紫外/可见光检测器））和Bio-Rad自动取样系统（AS-100型）、荧光计 Perkin Elmer型号LS 50 B“发光分光光度计”和分光光度计Hitachi U-1100分光光度计，带有样品吸管。这些系统将用于支持科罗拉多大学三个实验室的研究;所有这些实验室都参与分析和量化对生态过程很重要的各种化合物。在迪恩·鲍尔斯（Deane Bowers）教授的实验室中，研究重点是分析在三营养相互作用（植物-食草昆虫-天敌）中重要的植物和昆虫一级和二级化合物。Steve Schrnidt教授实验室的研究主要集中在两个领域。第一个项目是研究微生物对五氯苯酚的生物降解;第二个项目是与Russell蒙森教授的合作项目。这项合作研究在几个层面上研究高山营养循环：高山植物吸收氨基酸的过程和机制，以及与植物-微生物相互作用（包括真菌）相关的氨基酸吸收。这三个实验室的工作将因所需设备的提供而得到极大的加强，这些设备目前无法获得、不可靠和过时，或在其他实验室大量用于研究。

A plan is presented to study the mechanistic and ecological aspects of plant interactions with the abiotic environment; with microbes, including mycorrhizae; and with herbivorous insects. We request funds for a High Pressure Liquid Chromatography (HPLC) Workstation (Beckman Instrument Bioseparation Gradient HPLC "System Gold" (Model 126 Programmable Solvent Module and Model 166 Ultraviolet/Visible Detector) and a Bio-Rad Automatic Sampling System (Model AS-100) , a fluorometer Perkin Elmer model LS 50B "Luminescence Spectrometer" , and a spectrophotometer Hitachi U-1100 Spectrophotometer with sample sipper . These systems will be used to support research in three laboratories at the University of Colorado; all of which are involved in the analysis and quantification of a variety of chemical compounds important in ecological processes. In the laboratory of Professor Deane Bowers, research is focused on the analysis of plant and insect primary and secondary compounds that are important in tritrophic interactions (plants-herbivorous insects-natural enemies). Research in the laboratory of Professor Steve Schrnidt is in two major areas. In the first, biodegradation of pentachlorophenol (PCP) by microorganisms is studied; and the second is a collaborative project with Professor Russell Monson. This collaborative research studies alpine nutrient cycling at several levels: the processes and mechanisms of amino acid uptake by alpine plants, and amino acid uptake in relation to plant-microbe interactions, including mycorrhizae. The efforts of these three laboratories will be greatly enhanced by the availability ofthe requested equipment, which is presently unavailable, unreliable and out-of-date, or heavily used for research in other laboratories.