Analytical Chemical Analysis in an Ecological Context: a High Pressure Liquid Chromatography Workstation, Fluorometer and Spectrometer

提出了一项研究植物与非生物环境、微生物(包括菌根)和食草性昆虫相互作用的机制和生态学方面的计划。我们需要资金用于一台高压液相色谱(HPLC)工作站(Beckman仪器生物分离梯度高效液相色谱“系统金”(126型可编程溶剂模块和166型紫外线/可见光检测器)和一套Bio-Rad自动进样系统(AS-100型)、一台Perkin Elmer LS50B型荧光光谱仪和一台日立U-1100型分光光度计带进样器)。这些系统将用于支持科罗拉多大学三个实验室的研究；所有这些实验室都参与了对生态过程中重要的各种化合物的分析和量化。在迪恩·鲍尔斯教授的实验室里，研究重点是对植物和昆虫的初级和次级化合物进行分析，这些化合物在三营养相互作用(植物-草食性昆虫-天敌)中起着重要的作用。Steve Schrnidt教授的实验室研究主要在两个领域进行。第一个项目是研究微生物对五氯苯酚(PCP)的生物降解；第二个项目是与拉塞尔·蒙森教授的合作项目。这项合作研究在几个水平上研究高山营养循环：高山植物吸收氨基酸的过程和机制，以及与植物-微生物相互作用，包括菌根的氨基酸吸收。这三个实验室的努力将因所需设备的供应而大大加强，这些设备目前无法获得、不可靠和过时，或在其他实验室的研究中大量使用。

A plan is presented to study the mechanistic and ecological aspects of plant interactions with the abiotic environment; with microbes, including mycorrhizae; and with herbivorous insects. We request funds for a High Pressure Liquid Chromatography (HPLC) Workstation (Beckman Instrument Bioseparation Gradient HPLC "System Gold" (Model 126 Programmable Solvent Module and Model 166 Ultraviolet/Visible Detector) and a Bio-Rad Automatic Sampling System (Model AS-100) , a fluorometer Perkin Elmer model LS 50B "Luminescence Spectrometer" , and a spectrophotometer Hitachi U-1100 Spectrophotometer with sample sipper . These systems will be used to support research in three laboratories at the University of Colorado; all of which are involved in the analysis and quantification of a variety of chemical compounds important in ecological processes. In the laboratory of Professor Deane Bowers, research is focused on the analysis of plant and insect primary and secondary compounds that are important in tritrophic interactions (plants-herbivorous insects-natural enemies). Research in the laboratory of Professor Steve Schrnidt is in two major areas. In the first, biodegradation of pentachlorophenol (PCP) by microorganisms is studied; and the second is a collaborative project with Professor Russell Monson. This collaborative research studies alpine nutrient cycling at several levels: the processes and mechanisms of amino acid uptake by alpine plants, and amino acid uptake in relation to plant-microbe interactions, including mycorrhizae. The efforts of these three laboratories will be greatly enhanced by the availability ofthe requested equipment, which is presently unavailable, unreliable and out-of-date, or heavily used for research in other laboratories.