RUI: Conformation Analysis of Proteinaceous Bioadhesives

RUI：蛋白质生物粘合剂的构象分析

• 批准号: 9513390
• 负责人: Marion Olivieri
• 金额: $ 14.61万
• 依托单位: D'Youville College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9513390&HistoricalAwards=false
• 关键词: RUI; Conformation; Analysis; Proteinaceous; Bioadhesives

9513390 Olivieri This study seeks to elucidate the molecular structure(s) associated with the physical-chemical mechanism(s) whereby proteins bind to solid surfaces. To achieve the specific aim of the current proposal it is necessary to obtain data that will assist in the calculation of the three dimensional structures of known bioadhesives. Specifically, MAP extracted from the Blue Sea Mussel, (Mytilus edulis) will provide the model compounds. MAP is composed of 75(85 repeating decapeptide units with the primary sequence NH2-A-K-P-S-Y-HYP-HYP-T-DOPA-K-COOH (Waite et al., 1985). Since MAP is active at interfaces, it is therefore important to study its behavior while adsorbed to a surface. Solid-state techniques, such as multiple attenuated internal reflectance infrared (MAIR-IR) spectroscopy, ellipsometry, contact angle analysis, and solution-state nuclear magnetic resonance (NMR) spectroscopy, already have given some structural information about these peptides' abilities, but have not been used to their fullest potential. These techniques, in addition to crystallization efforts, will be employed to study the decameric repeat unit in addition to other MAP protein fragments (e.g., +NH3-proline-serine-tyrosine-hydroxyproline-hydroxyproline-thereonine-tyrosine-lysine-alanine-lysine-proline-serine-tryosine-hydroxyproline-COOH, and +NH3-proline-serine-tyrosine-hydroxyproline-hydroxyproline-threonine-3,4-dihydroxyphenylalanine-lysine-alanine-lysine-proline-serine-tyrosine-hydroxyproline-COOH). Data will be collected for the MAP peptides in both the solid-and solution-states, with the information compiled to create molecular models of the peptides. The first step of structure elucidation will be to use NMR data to create solution-state models. The variety of solid-state techniques would then be used to characterize the molecular changes that are responsible for the bioadhesion process. %%% The PI has worked to combine research with teaching. She takes her teaching responsibilities very s eriously, and is dedicated toward providing a learning environment for students that is thorough, yet inviting. The variety of courses the PI has taught at D'YC reflects her multi-disciplinary background (Introduction to Computers, Statistics, Biochemistry, General Chemistry I-II, Student Research I-II, and Chemistry for the Health Sciences II). She has prepared a 80+ page hand-out of lecture notes for the biochemistry class has co-authored a manual for the Chemistry for Health Sciences Laboratory and is working on a manual for the Biochemistry laboratory. To increase availability to the students, she conducts study sessions and distributes her home telephone number. Some students have attributed some of success to hand-outs distributed, graded homework and oral presentation assignments. A Biomaterials course will be developed that introduces implantology and describes the methods for choosing and characterizing materials for specific biological applications. The PI has already developed several new laboratory exercises for her biochemistry laboratory course and will prepare additional exercises that combine science disciplines. ***

9513390奥利维里本研究试图阐明与蛋白质与固体表面结合的物理化学机制有关的分子结构(S)(S)。为了实现本提案的具体目标，有必要获得有助于计算已知生物粘合剂的三维结构的数据。具体来说，从蓝海贻贝(Mytilus Edulis)中提取的MAP将提供模型化合物。MAP由75个(85个重复的十肽单元)组成，主序列为NH2-A-K-P-S-Y-HYP-HYP-T-DOPA-K-COOH(Waite等人，1985年)。由于MAP在界面上是活性的，因此研究其在表面吸附时的行为是很重要的。固体技术，如多重衰减内反射红外光谱(MAIR)、椭圆偏振光谱、接触角分析和溶液状态核磁共振光谱，已经给出了关于这些多肽能力的一些结构信息，但还没有被充分利用。除了结晶工作之外，这些技术还将被用于研究除其他MAP蛋白片段(例如+NH3-proline-serine-tyrosine-hydroxyproline-hydroxyproline-thereonine-tyrosine-lysine-alanine-lysine-proline-serine-tryosine-hydroxyproline-COOH，和+NH3-proline-serine-tyrosine-hydroxyproline-hydroxyproline-threonine-3，4-dihydroxyphenylalanine-lysine-alanine-lysine-proline-serine-tyrosine-hydroxyproline-COOH).)之外的十聚体重复单元将收集固态和溶液状态下的MAP多肽的数据，并汇编信息以创建多肽的分子模型。结构阐明的第一步将是使用核磁共振数据来创建溶液状态模型。然后，各种固态技术将被用来表征导致生物黏附过程的分子变化。PPI致力于将研究与教学相结合。她非常认真地承担起S的教学责任，致力于为学生提供一个彻底而又诱人的学习环境。PI在D‘YC教授的各种课程反映了她的多学科背景(计算机概论、统计学、生物化学、普通化学I-II、学生研究I-II和健康科学II化学)。她已经准备了80多页的生物化学课堂讲稿，为健康科学化学实验室合著了一本手册，并正在为生物化学实验室编写一本手册。为了增加学生的可获得性，她举办了学习课程，并分发了她的家庭电话号码。一些学生将一些成功归因于分发讲义、评分作业和口头报告作业。将开发一门生物材料课程，介绍植入学，并描述为特定生物应用选择和表征材料的方法。PI已经为她的生物化学实验室课程开发了几个新的实验室练习，并将准备结合科学学科的额外练习。***