SGER: Laser-Trapping -- A New Tool to Measure Rapid Physical Changes in Root Hairs Following Bacterial Attachment

9522043王这个项目建议应用一种名为“光学捕获”的新技术来研究大豆根细胞中与共生细菌日本慢生根瘤菌结合时的初始事件。“光学陷阱”利用聚焦的激光束将微米大小的粒子(S)捕获在焦平面内。利用光学捕捉器，可以将日本芽孢杆菌的细胞移动到大豆细胞的表面，以研究它们在根的特定区域内的单个大豆细胞上的附着效果，并明确结合的时间。将监测膜电位、pH、钙通量和细胞骨架组织随时间的变化。日本芽孢杆菌与大豆细胞的相互作用是具有生物学意义的配体(大豆细胞)与靶细胞(大豆细胞)结合的一个例子，系统地描述该系统中植物细胞与配体结合时的结构和生理动态可能为分析其他受体-配体和/或细胞黏附系统提供参考。***

9522043 Wang This project proposes to apply a new technology, designated "optical trapping," to study the initial events in a soybean root cell upon binding of symbiotic bacteria, Bradyrhizobium japonicum. The "optical trap" employs a focused beam of laser light to trap a micron-sized particle(s) within the focal plane. The use of the optical trap will allow B. japonicum cells to be moved to the surface of a soybean cell to study the effects of their adhesion on a single soybean cell in a defined region of the root, and with a clear definition of the time of binding. Time-dependent changes in membrane potential, pH, calcium fluxes, and cytoskeletal organization will be monitored. The B. japonicum - soybean cell interaction is an example of the binding of a biologically significant ligand (the B. japonicum cell) to a target (soybean cell), and a systematic description of the structural and physiological dynamics of the plant cell upon ligand binding in this system may offer insights on the analysis of other receptor-ligand and/or cell adhesion systems. ***