SGER: Laser-Trapping -- A New Tool to Measure Rapid Physical Changes in Root Hairs Following Bacterial Attachment

小行星9522043 本计画拟应用一种名为“光学捕捉”的新技术，来研究大豆根细胞中共生细菌大豆慢生根瘤菌（Bradyrhizobium japonicum）结合后的初始事件。 “光学陷阱”采用聚焦的激光束来捕获焦平面内的微米尺寸的颗粒。 光阱的使用将允许B。将大豆细胞移动到大豆细胞的表面，以研究它们在根的限定区域中对单个大豆细胞的粘附效果，并且具有结合时间的明确定义。 将监测膜电位、pH值、钙通量和细胞骨架组织的时间依赖性变化。 B。大豆-大豆细胞相互作用是生物学上重要的配体（B.大豆细胞）与靶标（大豆细胞）的结合，并且在该系统中配体结合后植物细胞的结构和生理动力学的系统描述可以提供对其他受体-配体和/或细胞粘附系统的分析的见解。 ***

9522043 Wang This project proposes to apply a new technology, designated "optical trapping," to study the initial events in a soybean root cell upon binding of symbiotic bacteria, Bradyrhizobium japonicum. The "optical trap" employs a focused beam of laser light to trap a micron-sized particle(s) within the focal plane. The use of the optical trap will allow B. japonicum cells to be moved to the surface of a soybean cell to study the effects of their adhesion on a single soybean cell in a defined region of the root, and with a clear definition of the time of binding. Time-dependent changes in membrane potential, pH, calcium fluxes, and cytoskeletal organization will be monitored. The B. japonicum - soybean cell interaction is an example of the binding of a biologically significant ligand (the B. japonicum cell) to a target (soybean cell), and a systematic description of the structural and physiological dynamics of the plant cell upon ligand binding in this system may offer insights on the analysis of other receptor-ligand and/or cell adhesion systems. ***