(SGER): Enzymatic Amplification of Genomic DNA

9522212韦斯 世界各地的人类学家在他们的实验室里拥有少量的人类组织，这些组织是在过去几十年的人口调查中收集的。 人们普遍对在世界范围内评估人类变异、其数量、模式和起源感兴趣;有人提议为此目的收集数据，称为人类基因组多样性项目。 对于任何这样的项目，重要的是要有来自世界各地样本的开放式数量的基因组DNA，以便许多研究人员可以广泛地分析它们。 该项目的目的是满足以低成本扩增来自实地收集或档案样本的代表性基因组DNA的开放式供应的需要，这可以由世界各地的实验室进行。 研究人员建议对扩增少量DNA的探索性方法进行系统的开发工作。 该方法（衔接子连接和扩增-AAA）涉及将基因组DNA切割成有用的长片段，并以特定的方式扩增这些片段，以便结果是含有尽可能多的原始基因组的混合物。 如果这种方法能够有效地发展，就可以产生大量的DNA，这些DNA很容易处理和储存，而且成本低廉。 这种方法如果成功，可以对人类基因组多样化项目以及其他大规模遗传调查的样本设计产生变革性影响。

9522212 WEISS Anthropologists around the world possess in their laboratories small amounts of human tissue collected in surveys of human populations over the past several decades. Widespread interest exists in a worldwide assessment of human variation, its amount, patterning and origins; an effort to collect data for this purpose, called the Human Genome Diversity Project (HGDP) has been proposed. For any such project, it will be important to have open-ended amounts of genomic DNA from worldwide samples, so that many investigators can analyze them extensively. The purpose of this project is to address the need for amplifying an open-ended supply of representative genomic DNA from field-collected or archival samples at a low cost, that can be undertaken by laboratories around the world. The investigators propose to do systematic developmental work on an exploratory method for amplifying small amounts of DNA. The method (Adapter Attachment and Amplification-AAA) involves cutting genomic DNA in usefully long fragments and amplifying those fragments in a specific way so that the result is a mix containing as much of the original genome as possible. If this method can be developed effectively, large quantities of DNA can be generated that are easy and inexpensive to work with and store. This approach, if successful, can have a transforming effect on the sample design of the HGDP as well as other large-scale genetic surveys.