(SGER): Enzymatic Amplification of Genomic DNA

在过去的几十年里，世界各地的人类学家在他们的实验室里拥有少量的人类组织，这些组织是在对人类种群的调查中收集的。在世界范围内对人类变异、其数量、模式和起源的评估存在着广泛的兴趣；为此目的而收集数据的努力被称为人类基因组多样性计划（HGDP）。对于任何这样的项目来说，从世界各地的样本中获得数量不限的基因组DNA是很重要的，这样许多研究人员就可以对它们进行广泛的分析。该项目的目的是为了满足以低成本扩大实地收集或档案样本中具有代表性的基因组DNA的无限制供应的需求，这可以由世界各地的实验室进行。研究人员建议对扩增少量DNA的探索性方法进行系统的开发工作。这种方法（适配器连接和扩增- aaa）包括将基因组DNA切割成有用的长片段，并以特定的方式扩增这些片段，以便结果是包含尽可能多的原始基因组的混合物。如果这种方法能够有效地发展，就可以产生大量的DNA，这些DNA易于处理和储存，而且价格低廉。这种方法，如果成功的话，可以对HGDP的样本设计以及其他大规模遗传调查产生转变作用。

9522212 WEISS Anthropologists around the world possess in their laboratories small amounts of human tissue collected in surveys of human populations over the past several decades. Widespread interest exists in a worldwide assessment of human variation, its amount, patterning and origins; an effort to collect data for this purpose, called the Human Genome Diversity Project (HGDP) has been proposed. For any such project, it will be important to have open-ended amounts of genomic DNA from worldwide samples, so that many investigators can analyze them extensively. The purpose of this project is to address the need for amplifying an open-ended supply of representative genomic DNA from field-collected or archival samples at a low cost, that can be undertaken by laboratories around the world. The investigators propose to do systematic developmental work on an exploratory method for amplifying small amounts of DNA. The method (Adapter Attachment and Amplification-AAA) involves cutting genomic DNA in usefully long fragments and amplifying those fragments in a specific way so that the result is a mix containing as much of the original genome as possible. If this method can be developed effectively, large quantities of DNA can be generated that are easy and inexpensive to work with and store. This approach, if successful, can have a transforming effect on the sample design of the HGDP as well as other large-scale genetic surveys.