(SGER): Enzymatic Amplification of Genomic DNA

世界各地的9522212位维斯人类学家在他们的实验室里拥有过去几十年在人口调查中收集到的少量人类组织。对人类变异、其数量、模式和起源的全球评估引起了广泛的兴趣；有人提出了一项为此目的收集数据的努力，称为人类基因组多样性项目(HGDP)。对于任何这样的项目，拥有来自世界各地的开放式数量的基因组DNA将是重要的，这样许多研究人员就可以广泛地分析它们。该项目的目的是满足从现场采集的或存档的样本中以低成本扩大代表性基因组DNA的不限成员名额供应的需要，这一需求可以由世界各地的实验室进行。研究人员提议对一种扩增少量DNA的探索性方法进行系统的开发工作。这种方法(适配器连接和扩增-AAA)涉及将基因组DNA切割成有用的长片段，并以特定的方式放大这些片段，从而得到包含尽可能多原始基因组的混合物。如果能够有效地开发这种方法，就可以产生大量的DNA，这些DNA易于处理和存储，而且成本低廉。这种方法如果成功，可能会对HGDP的样本设计以及其他大规模的基因调查产生转变作用。

9522212 WEISS Anthropologists around the world possess in their laboratories small amounts of human tissue collected in surveys of human populations over the past several decades. Widespread interest exists in a worldwide assessment of human variation, its amount, patterning and origins; an effort to collect data for this purpose, called the Human Genome Diversity Project (HGDP) has been proposed. For any such project, it will be important to have open-ended amounts of genomic DNA from worldwide samples, so that many investigators can analyze them extensively. The purpose of this project is to address the need for amplifying an open-ended supply of representative genomic DNA from field-collected or archival samples at a low cost, that can be undertaken by laboratories around the world. The investigators propose to do systematic developmental work on an exploratory method for amplifying small amounts of DNA. The method (Adapter Attachment and Amplification-AAA) involves cutting genomic DNA in usefully long fragments and amplifying those fragments in a specific way so that the result is a mix containing as much of the original genome as possible. If this method can be developed effectively, large quantities of DNA can be generated that are easy and inexpensive to work with and store. This approach, if successful, can have a transforming effect on the sample design of the HGDP as well as other large-scale genetic surveys.