Developing Adsorptive Protein Separations

CTS-9522528艾德莱特富特撒切尔鲁特威斯康星州麦迪逊大学 摘要本研究项目的重点是蛋白质色谱（吸附）分离关键参数的测量。NMR将用于获得颗粒内扩散系数，并开发吸附柱性能的预测相关性。 非unifopm流，无论是在颗粒尺寸的规模（连接分散），并在宏观水平（沟道），将使用磁共振成像（MRI）进行研究。 研究人员还将使用MRI检查高柱负荷下热力学和运输的相互作用。 预期的好处包括加强质量控制，降低蛋白质生产成本，缩短从药物发现到临床应用的开发时间。 这些好处将主要来自改进的设计程序，生物分离使用色谱法。

CTS-9522528 Ed Lightfoot Thatcher Root University of Wisconsin Madison ABSTRACT The research project focuses on the measurement of key parameters for chromatographic (adsorptive) separation of proteins. NMR will be used to obtain intraparticle diffusion coefficients and develop predictive correlations for the performance of the adsorption column. Non-unifopm flow, both on a scale of particle dimensions (connective dispersion) and at a macroscopic level (channeling), will be studied using magnetic resonance imaging (MRI). The investigators will also examine the interaction of thermodynamics and transport at high column loadings using MRI. Anticipated benefits include enhanced quality control, lower costs of manufacturing proteins and shorter development times from drug discovery to clinical application. These benefits will result primarily from improved design procedures for bioseparations using chromatography.