GOALI: Recovery of Recombinant Proteins from Transgenic Soybeans

9522644 Glatz这项建议正在研究中，目的是建立一个数据库，以帮助使用转基因大豆大规模生产重组蛋白。特别令人感兴趣的是从转基因大豆中生产和分离重组蛋白时涉及的降解和纯化过程。这项研究的基本目标包括两个主要活动：(1)确定常规大豆加工中的哪些步骤可能会影响转基因大豆重组蛋白的产量和活性；(2)研究利用“纯化融合”技术从大豆提取物中回收重组蛋白的可行性。后一种活性将通过添加蛋白质、β-葡萄糖醛酸酶和溶菌酶的实验来研究，以及使用实际转基因大豆的实验来研究，转基因大豆将在爱荷华州约翰斯敦的先锋高繁育国际公司提供的豆类中包含这些蛋白质和新霉素磷酸转移酶II蛋白。***

9522644 Glatz This proposal is on research to establish a data base to aid in the use of transgenic soy beans for the large-scale production of recombinant proteins. Of particular interest are the degradation and purification processes involved when producing and isolating a recombinant protein from transgenic soy beans. Basic goals of this research include two main activities: (1) to determine which steps in conventional soy bean processing are likely to influence the yield and activity of recombinant proteins from transgenic soy beans, and (2) to investigate the feasibility of using "purification fusion" technologies to aid in the recovery of recombinant proteins from soy bean extracts. The latter activity is to be investigated using experiments by adding the proteins, beta-glucuronidase and lysozyme, as well as experiments with actual transgenic soy which will contain these proteins along with the protein neomycin phosphotransferase II in beans supplied by Pioneer Hi-Bred International, Inc. located in Johnstown, Iowa. ***