GOALI: Recovery of Recombinant Proteins from Transgenic Soybeans

9522644 Glatz该提案旨在建立一个数据库，以帮助利用转基因大豆大规模生产重组蛋白。特别令人感兴趣的是从转基因大豆中生产和分离重组蛋白时涉及的降解和纯化过程。本研究的基本目标包括两个主要活动：(1)确定传统大豆加工中的哪些步骤可能影响转基因大豆重组蛋白的产量和活性；(2)研究使用“纯化融合”技术帮助从大豆提取物中回收重组蛋白的可行性。后一种活性将通过添加蛋白质、β -葡糖醛酸酶和溶菌酶的实验来研究，以及用实际的转基因大豆进行实验，这些大豆将含有这些蛋白质和蛋白质新霉素磷酸转移酶II，这些大豆由位于爱荷华州约翰斯敦的Pioneer Hi-Bred International公司提供。＊＊＊

9522644 Glatz This proposal is on research to establish a data base to aid in the use of transgenic soy beans for the large-scale production of recombinant proteins. Of particular interest are the degradation and purification processes involved when producing and isolating a recombinant protein from transgenic soy beans. Basic goals of this research include two main activities: (1) to determine which steps in conventional soy bean processing are likely to influence the yield and activity of recombinant proteins from transgenic soy beans, and (2) to investigate the feasibility of using "purification fusion" technologies to aid in the recovery of recombinant proteins from soy bean extracts. The latter activity is to be investigated using experiments by adding the proteins, beta-glucuronidase and lysozyme, as well as experiments with actual transgenic soy which will contain these proteins along with the protein neomycin phosphotransferase II in beans supplied by Pioneer Hi-Bred International, Inc. located in Johnstown, Iowa. ***