GOALI: Recovery of Recombinant Proteins from Transgenic Soybeans

9522644 Glatz该提案是关于建立数据库的研究，以帮助使用转基因大豆大规模生产重组蛋白。 特别感兴趣的是从转基因大豆中生产和分离重组蛋白时所涉及的降解和纯化过程。 本研究的基本目标包括两个主要活动：（1）确定常规大豆加工中哪些步骤可能影响转基因大豆重组蛋白的产量和活性，以及（2）研究使用“纯化融合”技术帮助从大豆提取物中回收重组蛋白的可行性。 后一种活性将使用通过加入蛋白质、β-葡糖醛酸糖苷酶和溶菌酶的实验，以及使用实际转基因大豆的实验来研究，所述实际转基因大豆将含有这些蛋白质以及由Pioneer Hi-Bred International，Inc.提供的豆类中的蛋白质新霉素磷酸转移酶II沿着。位于爱荷华州的约翰斯敦。 ***

9522644 Glatz This proposal is on research to establish a data base to aid in the use of transgenic soy beans for the large-scale production of recombinant proteins. Of particular interest are the degradation and purification processes involved when producing and isolating a recombinant protein from transgenic soy beans. Basic goals of this research include two main activities: (1) to determine which steps in conventional soy bean processing are likely to influence the yield and activity of recombinant proteins from transgenic soy beans, and (2) to investigate the feasibility of using "purification fusion" technologies to aid in the recovery of recombinant proteins from soy bean extracts. The latter activity is to be investigated using experiments by adding the proteins, beta-glucuronidase and lysozyme, as well as experiments with actual transgenic soy which will contain these proteins along with the protein neomycin phosphotransferase II in beans supplied by Pioneer Hi-Bred International, Inc. located in Johnstown, Iowa. ***