Design and Synthesis of CO2-Soluble Affinity Ligands and Surfactants for Use in Carbon Dioxide Extraction of Proteins

摘要埃里克·贝克曼艾伦·拉塞尔匹兹堡大学CTS-9523993 从细胞培养液中纯化蛋白质通常是一个涉及过滤和色谱步骤的多步骤过程。 从工艺的角度来看，特别是关于通量，将液-液萃取应用于蛋白质纯化将是有利的，但缺乏选择性和产物回收困难阻碍了大规模的这一点。 在过去十年的许多出版物中，Hatton及其同事以及其他人已经表明，表面活性剂可以用于将蛋白质从缓冲液中提取到有机相中，通常通过操纵水相的物理化学性质（pH值，离子强度等）。 以前，反胶束萃取蛋白质已被证明只有少数传统的液体烷烃，如己烷和异辛烷。

ABSTRACT Eric J. Beckman Alan J. Russell University of Pittsburgh CTS-9523993 Purification of proteins from cell broth is usually a multi-step process involving filtration and chromatographic steps. From a process standpoint, particularly with regards to throughput, it would be advantageous to apply liquid-liquid extraction to protein purification, yet lack of selectivity and product recovery difficulties preclude this on a large scale. In a number of publications spanning the last ten years, Hatton and colleagues, as well as others, have shown that surfactants can be employed to extract proteins from buffer to an organic phase, generally through manipulation of the physicochemical properties of the aqueous phase (pH, ionic strength, etc.). Previously, reverse micellar extraction of proteins has been demonstrated in only a few conventional liquid alkanes such as hexane and isooctane.