Analysis of Molecular Interactions Influencing Turnip Crinkle Virus Pathogenesis on Arabidopsis Thaliana

9531745萝卜皱缩病毒是一种植物病原体，已被证明在植物体内的运动和系统侵染需要两个小蛋白。蛋白质的特性将通过定点突变和这些突变体的功能特性相结合来继续。缺失分析将被用来确定核酸结合和合作所必需的蛋白质区域。我们将尝试将这两个功能域分开。对TCV突变体的研究也将继续进行，该突变体不会在某些植物中诱导过敏反应，尽管它像野生型一样复制和传播。%植物病毒是定义最好的植物病原体之一；它们的基因组很小，很容易进行遗传操作。了解它们在系统感染过程中如何在植物中移动的问题是重要的，这项工作应该增加我们对这一过程的了解。***

9531745 Wobbe Turnip crinkle virus, a plant pathogen, has been shown to require two small proteins for movement and systemic infection in plants. Characterization of the proteins will be continued by site-directed mutagensis coupled with functional characterization of such mutants. Deletion analysis will be used to determine regions of the protein which are necessary for nucleic acid binding and cooperatively. An attempt will be made to separate the two functional domains. Work will also continue with the TCV mutant which does not induce the hypersensitive response in certain plants although it replicates and spreads as does wildtype. %%% Plant viruses are among the best defined plant pathogens; they have small genomes and are easily genetically manipulated. The question of how they move through the plant in the course of systemic infection is important to understand and this work should add to our knowledge of that process. ***