Bioanalytical Separation Equipment for the Undergraduate Laboratories

该项目资助了一台高效液相色谱仪和一套毛细管电泳仪。这些生物分析分离仪器正在下列本科实验室中使用：生化与环境分析、仪器技术、生物化学实验室和独立研究。最初，学生们在生化和环境分析实验室接触到这些仪器，在那里他们对尿液中代谢重要的化合物或饮料中的人工甜味剂和添加剂进行高效液相分析。学生提供自己的样本进行分析。在毛细管电泳法上进行了一个简单的实验，以演示使用涂层毛细管分离蛋白质的能力。由溶菌酶、核糖核酸酶A、细胞色素C和肌红蛋白组成的蛋白质混合物可以很容易地用自由区毛细管电泳法分离。在仪器技术中，学生学习如何优化参数和条件，以实现这些仪器上的高分辨率分离。在生物化学实验室，学生通过一个蛋白质的项目研究被介绍到实验生物化学领域。高效液相色谱和毛细管电泳法的有效性对分离蛋白的制备和纯度分析的方法有很大影响。当正式课程之外的时间充裕时，学生也可以使用该仪器进行化学专业所需的各种独立研究项目。

This project funds a high-performance liquid chromatography (HPLC) apparatus and a capillary electrophoresis (CE) system. These bioanalytical separation instruments are being used in the following undergraduate laboratories: Biochemical and Environmental Analysis, Instrumental Techniques, Biochemistry Lab and Independent Study. Initially students are exposed to these instruments in the Biochemical and Environmental Analysis Lab, where they carry out HPLC analysis of metabolically important compounds in the urine or artificial sweeteners and additives in beverages. Students provide their own samples for the analysis. A simple experiment on CE is being introduced to demonstrate the power of protein separation using a coated capillary. A protein mixture consisting of lysozyme, ribonuclease A, cytochrome C, and myoglobin may be readily separated using free-zone capillary electrophoresis. In Instrumental Techniques, the students learn to optimize parameters and conditions to achieve high resolution for separations on these instruments. In Biochemistry Lab, students are introduced to the field of experimental biochemistry through a project study of a protein. The availability of HPLC and CE greatly affects the methods available for the preparation of the protein and the analysis of the purity of the isolated protein. As time becomes available outside of the formal courses, the instrument can also be used by students doing various independent research projects which are required of chemistry majors.