Development of Continuous Separation and Refinement Equipment for Colloidal Solution of Proteins Using Isoelectric Focusing

提出了一种新的利用等电聚焦连续分离蛋白质胶体溶液的方法。该装置由细胞组成，其细胞在两侧被膜分离，两性电解质用作缓冲试剂。以0.2wt%牛血清白蛋白和0.1wt%牛血红蛋白二元蛋白溶液为过滤样品，研究了电场强度和槽内流速对分离效率的影响。结果表明，在电场强度为12 v/cm、保留时间为23 mim的条件下，各蛋白质的分离效率均在95%以上，且两性电解质可回收作为缓冲液重复使用。从蛋白质的质量平衡出发，通过实验得到了预测连续等电聚焦装置分离效率的半理论方程。

A new continuous fractionation of colloidal solution of proteins is proposed using isoelectric focusing. The device consists of cells whose cell is separated by membranes at the both sides and the ampholytes are used as a buffer reagent. The costly ampholytes are recovered from the effluent by ultrafiltration and reused as buffer without decreasing the separation efficiency.An experimental study has been carried out to investigate the effect of electric field strength and flow rate in the cell on the separation efficiency in the case of using binary proteins solution, 0.2wt% bovine serum albumin and 0.1wt% bovine hemoglobin, as samples of the filtration. It was confirmed from the result that each protein was separated with efficiency above 95% under the condition of 12v/cm of electric field strength and 23mim of retention time, and the ampholytes were able to recover and reuse as buffer. Futhermore a semi-theoretical equation for predicting separation efficiency of the continuous isoelectric focusing apparatus was obtained experimentally based the mass balance of protein.