Enhancing Advanced Biology Investigations with Cell Culture Capabilities

生物系正在获取设备，以建立一个细胞培养实验室，用于细胞生物学、免疫学和血液学的高级本科生生物学课程和调查课程，(1)使目前的工作能够作为调查研究完成更多工作，(2)使更多类型的细胞培养工作得以进行。这些课程强调研究性的、动手的教学策略，向学生介绍基本的细胞培养技术，然后要求他们设计和进行后续实验，分析数据，并交流他们的结果。在细胞生物学中，学生能够培养他们的细胞系，以研究细胞对不同刺激的反应，产生动态变化和适应(1)细胞形态，(2)生长，(3)培养和转染率，以及(4)原代肝细胞培养和肝癌细胞系的血清蛋白质合成的比较。在免疫学和血液学中，学生能够解决免疫细胞(1)激活，(2)表面受体激活和信号转导，以及(3)细胞合作的问题。1200-1400-ft2细胞培养实验室将配备4个II级生物安全柜、6个CO2孵化器、倒相显微镜、离心机和相关设备，为16名学生的实验室提供专用实验室。这个实验室每年为大约50名高级生物学学生提供机会，通过极大地扩展目前可能的技术范围，对人类和动物细胞生物学进行详细的调查。

The Biology Department is obtaining equipment to establish a Cell Culture Laboratory for advanced, undergraduate biology courses in Cell Biology, Immunology and Hematology, and Investigations courses (1) to enable current work to be done much more as investigatory research and (2) to enable additional types of cell culture work to be undertaken. These courses emphasize an investigative, hands-on pedagogical strategy in which students are introduced to basic cell culture techniques, then required to design and conduct subsequent experiments, analyze data, and communicate their results. In Cell Biology, students are able to propagate their cell lines for investigations into cellular responses to varied stimuli producing dynamic changes and adaptations in (1) cell morphology, (2) growth, (3) plating and transfection efficiencies, and (4) comparisons of serum protein synthesis by primary hepatocyte cultures with hepatoma lines. In Immunology and Hematology, students are able to address questions of immune cell (1) activation, (2) surface receptor activation and signal transduction, and (3) cellular cooperation. A 1200-1400-ft2 Cell Culture Laboratory is being equipped with four Class II biological safety cabinets, six CO2 incubators, inverted phase microscopes, centrifuges, and related equipment to provide a dedicated laboratory for 16-student-capacity laboratories. This laboratory provides opportunities for approximately 50 upper-level biology students per year to develop detailed investigations into human and animal cell biology by greatly expanding the range of techniques currently possible.