Enhancing Advanced Biology Investigations with Cell Culture Capabilities

利用细胞培养能力加强高级生物学研究

• 批准号: 9551856
• 负责人: David DeHeer
• 金额: $ 4.75万
• 依托单位: Calvin University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9551856&HistoricalAwards=false
• 关键词: Enhancing; Advanced; Biology; Investigations; Cell

The Biology Department is obtaining equipment to establish a Cell Culture Laboratory for advanced, undergraduate biology courses in Cell Biology, Immunology and Hematology, and Investigations courses (1) to enable current work to be done much more as investigatory research and (2) to enable additional types of cell culture work to be undertaken. These courses emphasize an investigative, hands-on pedagogical strategy in which students are introduced to basic cell culture techniques, then required to design and conduct subsequent experiments, analyze data, and communicate their results. In Cell Biology, students are able to propagate their cell lines for investigations into cellular responses to varied stimuli producing dynamic changes and adaptations in (1) cell morphology, (2) growth, (3) plating and transfection efficiencies, and (4) comparisons of serum protein synthesis by primary hepatocyte cultures with hepatoma lines. In Immunology and Hematology, students are able to address questions of immune cell (1) activation, (2) surface receptor activation and signal transduction, and (3) cellular cooperation. A 1200-1400-ft2 Cell Culture Laboratory is being equipped with four Class II biological safety cabinets, six CO2 incubators, inverted phase microscopes, centrifuges, and related equipment to provide a dedicated laboratory for 16-student-capacity laboratories. This laboratory provides opportunities for approximately 50 upper-level biology students per year to develop detailed investigations into human and animal cell biology by greatly expanding the range of techniques currently possible.

生物系正在获得设备，以建立细胞培养实验室，用于细胞生物学，免疫学和血液学的高级本科生物学课程和调查课程（1）使当前的工作能够更多地作为澄清性研究和（2）使其他类型的细胞培养工作能够进行。 这些课程强调调查，动手教学策略，其中学生介绍了基本的细胞培养技术，然后要求设计和进行后续实验，分析数据，并传达他们的结果。 在细胞生物学中，学生能够繁殖他们的细胞系，以研究细胞对不同刺激的反应，从而产生动态变化和适应（1）细胞形态，（2）生长，（3）平板接种和转染效率，以及（4）通过原代肝细胞培养物与肝癌细胞系进行血清蛋白合成的比较。 在免疫学和血液学，学生能够解决免疫细胞（1）激活，（2）表面受体激活和信号转导，（3）细胞合作的问题。 一个1200-1400平方英尺的细胞培养实验室配备了四个二级生物安全柜，六个二氧化碳培养箱，反相显微镜，离心机和相关设备，为16个学生容量的实验室提供专用实验室。 该实验室每年为大约50名高级生物学学生提供机会，通过大大扩展目前可能的技术范围，对人类和动物细胞生物学进行详细的调查。