Confocal imaging plate reader for advanced cell biology applications

适用于高级细胞生物学应用的共焦成像读板机

• 批准号: RTI-2022-00404
• 负责人: Khursigara, Cezar
• 金额: $ 10.93万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=761151
• 关键词: Confocal; imaging; plate; reader; advanced

Cost-effective high throughput imaging is the cornerstone of modern cell biology laboratories. However, traditional fluorescence imaging plate readers lack the ability to resolve intricate cellular details in 3D, while confocal microscopes that function to screen samples in high throughput are prohibitively expensive. This application seeks to fund a Cytation C10 Spinning Disk Confocal Imaging Plate Reader to support advanced cell biology initiatives from microbiomes to embryos. The Cytation C10 is currently the only confocal imaging plate reader available and combines automated spinning disk confocal microscopy, widefield microscopy and multi-mode detection in one compact system. This combination of imaging modalities will enable our research groups to image and quantify complex cellular information to obtain meaningful results faster. The NSERC-funded research of the applicants focuses on cutting-edge aspects of cell biology. The applicants share a common challenge of obtaining high-resolution image datasets of complex cellular systems in 3D, rapidly while yielding quantitative information. The research themes include rapid imaging of morphology and protein localization in bacterial cells, visualizing outputs of chemical genetic screens, dissecting signalling and molecular pathways involved in bacterial-host interactions, and studying endocrine and neuronal development. This infrastructure is urgently needed as the lack of access to robust, high throughput multi-mode imaging has severely limited the scope of their research programs. The research projects of HQP are currently limited in scope and hampered by the need to access equipment at distant sites, or the use of inconsistent and time-consuming manual microscopy methods. The ability to perform large-scale screens rapidly and accurately is thus critically needed and will enable HQP to ask more ambitious research questions. The team of applicants and their HQP represent a range of backgrounds and reflect the diversity of Canadian society today.

高性价比的高通量成像是现代细胞生物学实验室的基石。然而，传统的荧光成像平板阅读器缺乏在3D模式下解析复杂细胞细节的能力，而用于高通量筛选样本的共焦显微镜价格昂贵得令人望而却步。这项申请旨在资助Cytation C10旋转磁盘共聚焦成像平板读取器，以支持从微生物到胚胎的先进细胞生物学倡议。Cytation C10是目前唯一可用的共焦成像板阅读器，在一个紧凑的系统中结合了自动旋转圆盘共焦显微镜、宽视场显微镜和多模式检测。这种成像方式的组合将使我们的研究小组能够对复杂的细胞信息进行成像和量化，以更快地获得有意义的结果。NSERC资助的申请者的研究重点是细胞生物学的尖端方面。申请者共同面临着一个共同的挑战，即快速获得复杂蜂窝系统的高分辨率3D图像数据集，同时产生定量信息。研究主题包括细菌细胞中形态和蛋白质定位的快速成像，可视化化学遗传屏幕的输出，解剖细菌-宿主相互作用中涉及的信号和分子通路，以及研究内分泌和神经元发育。由于缺乏强大的、高通量的多模式成像，严重限制了他们的研究计划的范围，因此迫切需要这种基础设施。目前，HQP的研究项目范围有限，而且由于需要进入远程地点的设备，或使用不一致和耗时的人工显微镜方法而受到阻碍。因此，快速准确地进行大规模筛选的能力是至关重要的，这将使HQP能够提出更雄心勃勃的研究问题。申请者团队及其HQP代表了一系列的背景，反映了当今加拿大社会的多样性。