RUI: Characterization of Virescent Mutants in Arabidopsis thaliana

RUI：拟南芥绿色突变体的表征

• 批准号: 9600532
• 负责人: Judith Brusslan
• 金额: $ 21.8万
• 依托单位: California State University-Long Beach Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9600532&HistoricalAwards=false
• 关键词: RUI; Characterization; Virescent; Mutants; Arabidopsis

9600532 Brusslan Undifferentiated cells of the plant meristem contain proplastids. When leaf development is initiated, proplastids develop into chloroplasts, the site of photosynthesis and carbon fixation. Although numerous mutations that alter the chloroplast have been isolated from a large number of plant species, none so far have led to a clear understanding of the molecular events involved in chloroplast development. Arabidopsis thaliana offers an opportunity to understand this process because genes identified by mutation can be cloned. Virescent mutants that display a delay in greening and hence a delay in chloroplast development, have been isolated in the model plant Arabidopsis thaliana. The goal of this research is to characterize these mutants biochemically and genetically in order to determine which mutants are likely to be defective in the signal transduction pathways that regulate chlorplast development. A second goal is to analyze the pigments and proteins of the light harvesting complexes (LHCs) of all mutants, regulatory or non-regulatory. The assembly of the LHCs has not been regorously investigated on a genetic level in higher plants. Chloroplasts are essential for life on earth because photosynthesis produces oxygen and consumes carbon dioxide. A clear understanding of the development of the organelle that performs this important function is important for agriculatural and global warming research.

[600532] Brusslan植物分生组织的未分化细胞含有前质体。当叶片发育开始时，原质体发育成叶绿体，这是光合作用和固定碳的场所。虽然已经从大量植物物种中分离出了许多改变叶绿体的突变，但迄今为止，还没有一种突变导致对叶绿体发育的分子事件有明确的认识。拟南芥提供了一个了解这一过程的机会，因为通过突变识别的基因可以被克隆。在模式植物拟南芥中分离出了一种绿色突变体，该突变体显示绿化延迟，因此叶绿体发育延迟。本研究的目的是对这些突变体进行生化和遗传学表征，以确定哪些突变体在调节叶绿体发育的信号转导途径中可能存在缺陷。第二个目标是分析所有突变体的光收集复合物（lhc）的色素和蛋白质，无论是调节性的还是非调节性的。高等植物中lhc的组装尚未在遗传水平上进行严格的研究。叶绿体对地球上的生命至关重要，因为光合作用产生氧气并消耗二氧化碳。清楚地了解执行这一重要功能的细胞器的发展对农业和全球变暖研究非常重要。