CAREER: Identification of the Site-of-Action in Wheat of Pyrenophora tritici-repentis Toxin

9600914 Ciuffetti研究：这是一个职业奖。现代植物生物学的一个主要目标是对调节植物-微生物相互作用的因素进行分子定义。国际小麦局正在调查一种名为小麦褐斑病的疾病，这种疾病是由真菌Prenophora tritici-epentis引起的。这种真菌是致病的，因为它产生一种称为PTR毒素的宿主选择毒素(HST)。与所有其他HST不同，PTR毒素是一种蛋白质，是单个基因的直接产物。因此，这种真菌的致病力是由单个基因调节的，以产生毒素。此外，对PTR毒素敏感性的遗传评价表明，小麦对PTR毒素的反应和对病原菌的敏感性，是由单个显性基因控制的。该奖项的研究部分由四个具体目标组成，涵盖了多种方法，包括免疫学、生化和遗传学方法来鉴定PTR毒素的作用部位：目标1.对PTR毒素敏感的二倍体小麦的鉴定和遗传特性。目的2.PTR毒素作用部位/受体标记衍生物的制备及生化鉴定。目的3.利用酵母双杂交系统对毒素与其作用部位/受体之间的相互作用进行遗传学分析。目的4.PTR毒素作用部位/受体的免疫细胞化学定位。教育：在植物科学领域发展有效的学术生涯需要将研究和教育结合起来。俄勒冈州立大学和植物学和植物病理学系致力于这一整合。为了与大学和系的使命保持一致，即研究生和本科生的培训，招募未被充分代表的个人进入科学界，以及向社会推广，教育职业计划由四个主要目标组成：目标1.将VIDEOM ICROSCOPY、计算机增强成像和信息技术纳入本科植物科学教学。目标2.培养研究生成为更有效的植物科学教育者。目标3.对妇女的承诺和对本科生从事科学职业的培训。目标4.服务社会。该研究所的研究项目为教育活动提供了潜在的科学课题。

9600914 Ciuffetti Research: This is a CAREER Award. A major goal of modern plant biology is a molecular definition of factors that regulate plant-microbe interactions. The PI is investigating a disease called tan spot of wheat which is caused by the fungus, Pyrenophora tritici-repentis. This fungus is pathogenic because it produces the host-selective toxin (HST) called Ptr toxin. Unlike all other HSTs, Ptr toxin is a protein and the direct product of a single gene. Thus, pathogenicity in this fungus is regulated by a single gene for toxin production. Also, genetic evaluation of sensitivity to Ptr toxin has demonstrated that the wheat response to the toxin and consequently, susceptibility to the pathogen, is controlled by a single dominant gene. The research component of this award is composed of four specific objectives which encompass a diversity of approaches including an immunological, biochemical, and genetical approach to the identificaiton of the site-of-action of Ptr toxin: Objective 1. IDENTIFICATION AND GENETIC CHARACTERIZATION OF DIPLOID WHEATS SENSITIVE TO PTR TOXIN. Objective 2. PRODUCTION OF LABELED DERIVATIVES AND BIOCHEMICAL IDENTIFICATION OF THE SITE-OF- ACTION/RECEPTOR OF PTR TOXIN. Objective 3. GENETICAL ANALYSIS OF INTERACTIONS BETWEEN THE TOXIN AND ITS SITE-OF-ACTION/RECEPTOR USING THE YEAST TWO-HYBRID SYSTEM. Objective 4. IMMUNOCYTOCHEMICAL LOCALIZATION OF THE SITE-OF- ACTION/RECEPTOR OF PTR TOXIN. Education: Development of an effective academic career in the plant sciences requires integration of both research and education. Oregon State University and the Department of Botany and Plant Pathology are committed to this integration. In keeping with the mission of the University and the Department toward graduate and undergraduate training, the recruitment of underrepresented individuals into science, and outreach to the community, the education career plan is composed of four main objectives: Objective 1. INCORPORATION OF VIDEOM ICROSCOPY, COMPUTER ENHANCED IMAGING AND INFORMATION TECHNOLOGIES IN TEACHING UNDERGRADUATE PLANT SCIENCES. Objective 2. TRAINING GRADUATE STUDENTS TO BE MORE EFFECTIVE EDUCATORS IN THE PLANT SCIENCES. Objective 3. COMMITMENT TO WOMEN AND TRAINING OF UNDERGRADUATES FOR A CAREER IN THE SCIENCES. Objective 4. SERVICE TO THE COMMUNITY. This PI's research projects provide the underlying scientific subject for the education activities.