Isolation and identification of Tenacibaculum bacteria from a Chinook Salmon saltwater production site

在包括加拿大在内的世界各地的沿海海洋环境中都发现了藤杆菌细菌。在大西洋鲑鱼养殖中，有几种细小杆菌是条件致病菌，然而，对这种细菌的海洋生态以及太平洋三文鱼中细小杆菌的感染情况了解很少。该项目旨在识别不列颠哥伦比亚省奇努克鲑鱼咸水生产地及其周围的Tenacbaculum细菌。该项目将建立在圭尔夫大学(UGuelph)、温哥华岛大学(VIU)、创意鲑鱼有限公司和不列颠哥伦比亚省水生健康科学中心(CAHS)之间的牢固合作伙伴关系基础上。该项目的目标是：1)收集和测试Chinook鲑鱼网围水域及其周围的水样；2)在海水生产过程中收集和测试Chinook鲑鱼组织中的Tenacbaculum物种；3)收集和测试咸水生产场地的生物(无脊椎动物)和非生物(网围基础设施)生物和表面；4)利用高通量分子分析来调查Tenacbaculum物种的群落分布和收集样本中的相对丰度。这个项目将提供对更广泛的海洋环境中Tenacbaculum物种存在的了解，并有助于我们了解这种细菌在太平洋鲑鱼中定居的潜力。

Tenacibaculum bacteria are found in coastal marine environments worldwide, including Canada. Several Tenacibaculum species have been shown to be opportunistic pathogens on cultured Atlantic salmon, however, there is a limited understanding of the marine ecology of this bacteria and of Tenacibaculum infections in Pacific salmon. This project aims to identify Tenacibaculum bacteria within and surrounding a Chinook salmon saltwater production site in British Columbia. The project will build on a strong partnership between University of Guelph (UGuelph), Vancouver Island University (VIU), Creative Salmon Company Ltd. and the BC Center for Aquatic Health Sciences (CAHS). The objectives of this project are to 1) collect and test water samples for Tenacibaculum species in and around Chinook salmon saltwater net-pen site; 2) collect and test Chinook salmon tissue for Tenacibaculum species during saltwater production; 3) collect and test biotic (invertebrates) and abiotic (net-pen infrastructure) organisms and surfaces at the saltwater production site for Tenacibaculum species and 4) utilize a high-throughput molecular assay to investigate Tenacibaculum species community distribution and relative abundance in collected samples. This project will provide an understanding of the presence of Tenacibaculum species in the wider marine environment and aide in our understanding for the potential of the bacteria to colonise pacific salmon.