Acquisition of Equipment for Image Analysis and 3-D Reconstruction

这项提议用于购置设备，以支持使用图像分析的两个设施：一个图像分析核心设施和一个三维重建设施。这些设施将被安置在一座新的8万平方英尺的网络生物大楼的三楼和四楼的单独空间中。图像分析核心设施将能够获取宏观和微观图像。该设施的设备包括摄像机支架、立体显微镜、CCD摄像机、图像采集卡和装有Image Pro Plus软件的奔腾计算机、视频监视器和新奥林巴斯BH40荧光显微镜的隔振台。一台便携式X光机(安装在研究实验室中)将被用来产生关于活着的爬行动物骨骼大小和角度以及蛋的大小和数量的X射线图像。射线照片的测量将通过图像分析系统进行分析。数据将存储在Iomega 100 MB Zip驱动器上。一台装有Image Pro Plus和Zip驱动器的单独的奔腾电脑将安装在一个计算机实验室中，以便对存档的图像进行分析。这个设施将支持几个学科的几个生物学教员的研究项目。罗杰·安德森和赫布·布朗将利用这一设施进行个人和合作项目，以检查野生爬行动物的骨骼元素和生态性能。吉赛尔·穆勒·帕克和艾米丽·皮尔将使用图像分析来计算自发荧光藻类和荧光染色细菌的数量和大小。琼·罗斯将使用图像分析来测量苔藓虫化石的形态特征。三维重建设备将用于荧光染色胚胎的光学切片、散焦荧光的去除、三维结构的重建和三维数据的分析。设备包括徕卡DMRB显微镜的隔振台、高分辨率40倍油物镜、用于三维重建的CellScan系统、奔腾计算机、Tektronics打印机和用于数据存储的lGBJaz驱动器。这个设施将支持两个生物系的研究项目。David Leaf将使用三维设备对海胆胚胎中的Rab GTP结合蛋白进行免疫荧光定位。Mary Anne Pultz将使用该设备对具有针对果蝇发育调节蛋白的交叉反应抗体的Nasonia野生型和突变胚胎进行免疫荧光染色。这两个项目都得到了NIH地区赠款的支持。该设施还将支持Mary Anne Pultz和David Leaf的合作项目，通过原位杂交和免疫荧光染色分析Caudal的Nasonia同源物的基因表达。最后，该设施将被用来启动一个长期项目，即制作一份果蝇和纳斯尼亚的基因表达的比较三维图谱。

This proposal is for the acquisition of equipment to support two facilities using image analysis; an image analysis core facility and a three-dimensional reconstruction facility. These facilities will be housed in separate spaces on the third and fourth floors in a new 80,000 square foot networked Biology Building. The image analysis core facility will be able to acquire macroscopic and microscopic images. Equipment for this facility include a camera stand, stereo microscope, CCD camera, framegrabber and Pentium computer with Image Pro Plus software, video monitor, and vibration isolation table for a new Olympus BH40 epifluorescent microscope. A portable x-ray machine (housed in a research lab) will be used to produce x-ray images of bone sizes and angles and egg size and number in living reptiles. Radiographs measurements will be taken and analyzed with the image analysis system. Data will be stored on Iomega 100 MB zip drives. A separate Pentium computer with Image Pro Plus and Zip drive will be housed in a computer lab to allow analysis of archived images. This facility will support research programs of several Biology faculty across several disciplines. Roger Anderson and Herb Brown will be using this facility for individual and collaborative projects to examine skeletal elements and ecological performance in reptiles from the wild. Giselle Muller Parker and Emily Peele will use image analysis to count and size autofluorescent algae and fluorescently-stained bacteria. June Ross will use the image analysis to measure morphological features of fossil bryozoans. The three-dimensional reconstruction facility will be used for the optical sectioning of fluorescently-stained embryos, the removal of out-of-focus fluorescence, the reconstruction of three-dimensional structures, and the analysis of three-dimensional data. Equipment include a vibration isolation table for a Leica DMRB rnicroscope, a high resolution 40X oil objective, CellScan System for three-dimensional reconstructions, Pentium computer, Tektronics printer, and a lGBJaz drive for data storage. This facility will support research programs of two Biology faculty. David Leaf will use the three-dimensional facility for immunofluorescent localization of a rab GTPbinding protein in sea urchin embryos. Mary Anne Pultz will use the facility for immunofluorescent stainings of Nasonia wild type and mutant embryos with cross-reactive antibodies against Drosophila developmental regulatory proteins. Both projects are supported by NIH-AREA grants. This facility will also support a collaborative project of Mary Anne Pultz and David Leaf to analyze gene expression of the Nasonia homologue of Caudal by in situ hybridization and immunofluorescent staining. Finally, the facility will be used to start a long-term project on producing a comparative three-dimensional atlas of gene expression in Drosophila and Nasonia.