Acquisition and Development of High Resolution Digital Imaging Facility for the Investigation of Cytoskeletal and Membrane Protein Molecular Dynamics

项目摘要 仪器要求支持耶鲁大学细胞运动和细胞骨架组的四个实验室的互动研究工作。该提案旨在补充正在进行的合作研究工作，并扩大我们在分子动力学和共聚焦激光扫描显微镜领域的技术能力。具体而言，我们已要求获得资金的国家的最先进的共聚焦激光扫描显微镜和必要的组件，用于设计和建设高分辨率的数字成像系统，研究分子动力学。该系统将执行：荧光光漂白和光活化分析、单光束梯度激光捕获、细胞消融和荧光探针的真实的实时定量低光水平成像。 该奖项促进的研究活动将包括神经元生长和目标识别的分子动力学研究（Forscher）;肌动蛋白和微管分子马达的分子和功能分析（Mooseker，Wolenski和Rosenbaum）。所要求的仪器还将大大加强生物学系成像训练和研究设施的研究和训练使命。这包括在本科和研究生阶段的光学方法及其在分子细胞生物学中的应用的高级课程。

Project Summary Instrumentation is requested to support the interactive research efforts of the four laboratories comprising the Yale Cell Motility and Cytoskeletal Group. This proposal is aimed at complementing ongoing collaborative research efforts and extending our technical capabilities in the area of molecular dynamics and confocal laser scanning microscopy. Specifically, we have requested funding for acquisition of a state-of-the-art confocal laser scanning microscope and requisite components for design and construction of a high resolution digital imaging system for studying molecular dynamics. This system will implement: fluorescence photobleaching and photoactivation analyses, single beam gradient laser trapping, cell ablation and quantitative low light level imaging of fluorescent probes in real time. Research activities facilitated by this award will include investigation of the molecular dynamics of neuronal growth and target recognition (Forscher); molecular and functional analyses of actin and microtubule-based molecular motors (Mooseker, Wolenski and Rosenbaum). The requested instrumentation will also significantly augment the research and training mission of the Department of Biology's Training and Research Facility for Imaging. This includes advanced courses in optical methods and their use in molecular cell biology at both undergraduate and graduate levels.