RUI: Characterization of Cystathionine gamma-Synthase in Soybean

RUI：大豆中胱硫醚γ-合酶的表征

• 批准号: 9602190
• 负责人: Cleo Hughes-Darden
• 金额: $ 16万
• 依托单位: Morgan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-15 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9602190&HistoricalAwards=false
• 关键词: RUI; Characterization; Cystathionine; gamma; Synthase

9602190 Hughes The long term goal of this proposal is to thoroughly understand the regulatory mechanisms controlling the biosynthesis of the essential amino acid, methionine, in plants, especially soybean that has a low methionine content. This knowledge may enable us to determine how to alter these regulatory mechanisms to develop nutritionally improved plants that produce increased levels of methionine. The major goals are focussed on an important enzyme involved in methionine biosynthesis, cystationine gamma-synthase (CS), and its gene and/or gene family in soybean. CS is the branch point enzyme leading to methionine production from aspartate. In order, to understand the molecular and metabolic mechanisms that regulate methionine systhesis, the following specific aims will be addressed: I. What is the role of cystathionine gamma-synthase in methionine systhesis? II. What regulates the expression of the gene encoding cystathionine gamma-synthase? Plants are the major source of dietary proteins for umans and livestock. The essential amino acid content of these dietary proteins is very important because these amino acids are necessary to ensure proper growth in humans and livestock that are unable to synthesize them. Therefore, these amino acids are usually supplemented into their diets.

9602190 Hughes该提案的长期目标是彻底了解控制植物，特别是具有低甲硫氨酸含量的大豆中必需氨基酸甲硫氨酸生物合成的调节机制。 这一知识可能使我们能够确定如何改变这些调节机制，以开发营养改善的植物，产生更高水平的蛋氨酸。 主要目标集中在大豆中参与甲硫氨酸生物合成的重要酶--胱胺酸γ合成酶（CS）及其基因和/或基因家族。 CS是导致从天冬氨酸产生甲硫氨酸的分支点酶。 为了了解调节甲硫氨酸合成的分子和代谢机制，将解决以下具体目标： I.胱硫醚γ-合酶的作用是什么 在蛋氨酸合成中？ 二.是什么调控了编码 胱硫醚γ-合酶 植物是人类和家畜膳食蛋白质的主要来源。 这些膳食蛋白质的必需氨基酸含量非常重要，因为这些氨基酸是确保人类和牲畜正常生长所必需的，而人类和牲畜无法合成这些氨基酸。 因此，这些氨基酸通常补充到他们的饮食中。