A High Speed Centrifuge for Preparation of Biological Materials

一种用于生物材料制备的高速离心机

• 批准号: 9602940
• 负责人: Stephanie Aamodt
• 金额: $ 2.13万
• 依托单位: Louisiana State University Shreveport
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-15 至 1998-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9602940&HistoricalAwards=false
• 关键词: Speed; Centrifuge; Preparation; Biological; Materials

Funding is requested for the purchase of a floor-model, refrigerated high speed centrifuge and four rotors. The centrifuge would replace the one preparative centrifuge currently owned by Louisiana State University at Shreveport, a sixteen-year old Beckman J2-21 centrifuge. A high speed centrifuge is required for the routine preparation of proteins, nucleic acids and organelles for research experiments by six faculty members in the Departments of Biological Sciences and Chemistry and Physics. The centrifuge is also necessary for the preparation of materials for graduate and undergraduate student research and for laboratory courses. The specific instrument requested is the Beckman Avanti J-25I High Performance Centrifuge. Four rotors are desired to permit necessary centrifugation procedures: a large volume rotor (to hold six 500 ml bottles), a high speed rotor for 50 ml tubes (to 75,600 x g), a low-speed swinging bucket rotor that accommodates interchangeable buckets and adapters for multiple purposes and a swinging bucket rotor capable of forces to 26,500 x g.

需要资金购买落地式冷冻高速离心机和四个转子。该离心机将取代路易斯安那州立大学什里夫波特分校目前拥有的一台制备型离心机，即一台已有 16 年历史的 Beckman J2-21 离心机。 生物科学系、化学系和物理系的六名教员需要使用高速离心机来常规制备蛋白质、核酸和细胞器，以进行研究实验。离心机对于准备研究生和本科生研究以及实验室课程的材料也是必需的。所需的具体仪器是 Beckman Avanti J-25I 高性能离心机。需要四个转子来实现必要的离心程序：一个大容量转子（可容纳六个 500 ml 瓶子）、一个用于 50 ml 试管（至 75,600 x g）的高速转子、一个低速吊桶转子（可容纳用于多种用途的可互换吊桶和适配器）以及一个能够承受 26,500 x g 力的吊桶转子。