A High Speed Centrifuge for Preparation of Biological Materials

要求提供资金用于购买一台落地式冷冻高速离心机和四个转子。该离心机将取代路易斯安那州立大学目前在什里夫波特拥有的一台制备型离心机，一台有16年历史的贝克曼J2-21离心机。 生物科学系、化学系和物理系的六名教员需要一台高速离心机来进行蛋白质、核酸和细胞器的常规制备。离心机也是必要的准备材料的研究生和本科生的研究和实验室课程。所要求的特定仪器是Beckman Avanti J-25 I高性能仪器。需要四个转子以允许必要的离心程序：一个大容量转子（以容纳六个500 ml瓶），一个用于50 ml管的高速转子（至75，600 x g），一个低速摆桶转子（可容纳可互换的桶和适配器用于多种用途）和一个能够施加26，500 x g力的摆桶转子。

Funding is requested for the purchase of a floor-model, refrigerated high speed centrifuge and four rotors. The centrifuge would replace the one preparative centrifuge currently owned by Louisiana State University at Shreveport, a sixteen-year old Beckman J2-21 centrifuge. A high speed centrifuge is required for the routine preparation of proteins, nucleic acids and organelles for research experiments by six faculty members in the Departments of Biological Sciences and Chemistry and Physics. The centrifuge is also necessary for the preparation of materials for graduate and undergraduate student research and for laboratory courses. The specific instrument requested is the Beckman Avanti J-25I High Performance Centrifuge. Four rotors are desired to permit necessary centrifugation procedures: a large volume rotor (to hold six 500 ml bottles), a high speed rotor for 50 ml tubes (to 75,600 x g), a low-speed swinging bucket rotor that accommodates interchangeable buckets and adapters for multiple purposes and a swinging bucket rotor capable of forces to 26,500 x g.