Genetic Analysis of the Drosophila Immune Response

果蝇免疫反应的遗传分析

• 批准号: 9604323
• 负责人: Kathryn Anderson
• 金额: $ 37.5万
• 依托单位: Sloan Kettering Institute For Cancer Research
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604323&HistoricalAwards=false
• 关键词: Genetic; Analysis; Drosophila; Immune; Response

9604323 Anderson Technical The long-term goal of the project is to characterize the signaling pathways that are activated in Drosophila in response to infection by pathogens and to learn how the signaling pathways are coordinated between different tissues. In this proposal, a set of third chromosomal mutants that block specific aspects of the immune response will be characterized and one gene that affects multiple aspects of the immune response, ird1, will be characterized molecularly. 1. The ird1 gene will be cloned and its expression in hemocytes, fat body and other larval cell types assayed. Genetic mosaics will be constructed to determine whether ird1 affects multiple branches of the immune response because it coordinates these responses or whether it is required in each of the cell types. To determine what signals activate ird1 and how ird1 implements the immune response, genetic interactions of ird1 with other mutants response will be tested. 2. The genetic pathways that control specific branches of the immune response and coordinate the immune responses of different cell types will be defined. Complementation and mapping experiments will define the number and location of third chromosomal genes that control the immune response. The phenotypes of the novel genes affecting immune response will be characterized and compared with the phenotypes of previously-identified genes that may affect the immune response. Non-technical In all animals, the first rapid response to infection by pathogens is mediated by the innate immune response. Both the general strategies used in the innate immune response and a number of molecular mechanisms appear to be conserved between invertebrates and vertebrates. This is a proposal to initiate a systematic genetic study of the innate immune response in Drosophila. These experiments will identify genes required for the recognition of pathogen, for triggering the tissue-specific responses to pathogen and for coordinating the immune responses of diff erent tissues.

9604323安德森技术该项目的长期目标是确定果蝇在受到病原体感染时被激活的信号通路的特征，并了解不同组织之间信号通路是如何协调的。在这项提案中，将对一组阻止免疫反应特定方面的第三条染色体突变进行鉴定，并对影响免疫反应多个方面的一个基因ird1进行分子鉴定。1.克隆ird1基因并检测其在血细胞、脂肪体等幼虫细胞中的表达。将构建遗传马赛克，以确定ird1是否影响免疫反应的多个分支，因为它协调这些反应，或者是否每种细胞类型都需要ird1。为了确定什么信号激活ird1以及ird1如何实现免疫反应，将测试ird1与其他突变反应的遗传相互作用。2.将定义控制免疫反应的特定分支和协调不同细胞类型的免疫反应的遗传途径。互补和作图实验将确定控制免疫反应的第三条染色体基因的数量和位置。影响免疫反应的新基因的表型将被表征，并与先前识别的可能影响免疫反应的基因的表型进行比较。在所有非技术性动物中，对病原体感染的第一个快速反应是由先天免疫反应调节的。在先天免疫反应中使用的一般策略和一些分子机制似乎在无脊椎动物和脊椎动物之间都是保守的。这是一项启动对果蝇先天免疫反应的系统遗传学研究的提议。这些实验将确定识别病原体、触发对病原体的组织特异性反应以及协调不同组织的免疫反应所需的基因。