Sea Urchin Larval Development

海胆幼体发育

• 批准号: 9604454
• 负责人: R. Andrew Cameron
• 金额: $ 27万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-15 至 2000-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604454&HistoricalAwards=false
• 关键词: Sea; Urchin; Larval; Development

Cameron 9604454 In this project, Dr. Cameron examines the development of indirect developing sea urchin larvae, a life stage that arises from the embryo after the onset of feeding and bears little morphological resemblance to the adult into which it will eventually metamorphose. He focuses particularly though not exclusively on those larval developmental processes that lead to the formation of adult primordial structures. He will employ modern techniques of confocal microscopy, blastomere injection, lineage tracing, in situ hybridization and genetic chimeras to illustrate the developmental mechanism that lead to the formation of larval structures. The studies that he proposes fall into the following categories: 1) The mapping of set aside cell populations through the use of vital dyes, DNA stains and bromodeoxyuridine incorporation. 2) Experiments to alter larval division patterns and to test the cell number regulation in structures stemming from set aside cells. 3) The contribution of isolated animal-half embryos, vegetal-half embryos and the skeletogenic cells to adult structures and the larval primordia from which they arise using genetic chimeras detected by genomic microsatellite markers. 4) The examination of expression in larvae of genes likely to be involved in the formation of adult structures. 5) Observations on the adult rudiment to determine the earliest incidence of pentameral symmetry and the manner in which it is established.

卡梅隆9604454在这个项目中，卡梅伦博士研究了间接发育的海胆幼虫的发育，这是一个从开始摄食后的胚胎开始发育的生命阶段，在形态上与最终蜕变成鱼的成虫几乎没有相似之处。他特别关注那些导致成虫原始结构形成的幼虫发育过程，尽管不是唯一的。他将使用共聚焦显微镜、卵裂球注射、谱系追踪、原位杂交和遗传嵌合体等现代技术来阐明导致幼虫结构形成的发育机制。他建议的研究分为以下几类：1)通过使用生命染料、DNA染色和溴脱氧尿嘧啶核苷掺入来绘制留出的细胞群体图。2)改变幼虫分裂模式的实验，并测试从保留细胞产生的结构中细胞数量的调节。3)利用基因组微卫星标记检测的遗传嵌合体，研究分离的动物半胚胎、植物半胚胎和骨骼细胞对成体结构和幼虫原基的贡献。4)检测幼虫中可能参与成虫结构形成的基因的表达。5)对成体雏形进行观察，以确定最早出现的五核对称性及其形成方式。