Sea Urchin Larval Development

在这个项目中，卡梅隆博士研究了间接发育的海胆幼虫的发育，这是一个生命阶段，从开始进食后的胚胎开始，与最终蜕变的成虫在形态上几乎没有相似之处。他特别关注那些导致成虫原始结构形成的幼虫发育过程。他将使用共聚焦显微镜、卵裂球注射、谱系追踪、原位杂交和遗传嵌合体等现代技术来说明导致幼虫结构形成的发育机制。他提出的研究分为以下几类：1)通过使用活性染料、DNA染色和溴脱氧尿苷结合来绘制留出的细胞群。2)实验改变幼虫的分裂模式和测试细胞数量调节结构源于备用细胞。3)利用基因组微卫星标记检测基因嵌合体，研究分离的动物半胚胎、植物半胚胎和成骨细胞对成虫结构和幼虫原基形成的贡献。4)检测可能参与成虫结构形成的基因在幼虫中的表达。(5)对成年基础的观察，以确定五体对称最早发生的情况及其建立的方式。

Cameron 9604454 In this project, Dr. Cameron examines the development of indirect developing sea urchin larvae, a life stage that arises from the embryo after the onset of feeding and bears little morphological resemblance to the adult into which it will eventually metamorphose. He focuses particularly though not exclusively on those larval developmental processes that lead to the formation of adult primordial structures. He will employ modern techniques of confocal microscopy, blastomere injection, lineage tracing, in situ hybridization and genetic chimeras to illustrate the developmental mechanism that lead to the formation of larval structures. The studies that he proposes fall into the following categories: 1) The mapping of set aside cell populations through the use of vital dyes, DNA stains and bromodeoxyuridine incorporation. 2) Experiments to alter larval division patterns and to test the cell number regulation in structures stemming from set aside cells. 3) The contribution of isolated animal-half embryos, vegetal-half embryos and the skeletogenic cells to adult structures and the larval primordia from which they arise using genetic chimeras detected by genomic microsatellite markers. 4) The examination of expression in larvae of genes likely to be involved in the formation of adult structures. 5) Observations on the adult rudiment to determine the earliest incidence of pentameral symmetry and the manner in which it is established.