Pattern recognition receptors: discovery, function and applications in crops for durable disease control (PRR-CROP)

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Recent work in the model plant Arabidopsis has led to the discovery of pattern recognition receptors (PRRs) which recognise broad classes of pathogen molecules and offer the prospect of developing broad-spectrum disease control. The novel aspect of our proposal is to develop this knowledge so that it can be applied to crop plant species. We concentrate on cereals, brassica and grapevine, and focus on PRRs that recognise the fungi and oomycetes which are the major pathogens of these crops. The objectives of the proposal are:A) Identification and testing of novel bacterial, fungal and oomycete pathogen-associated molecular patterns (PAMPs) that are recognised by Arabidopsis and selected crops, e.g. barley, wheat, tomato, grapevine, brassica (P1, P2, P3, P4 and AP1). ). We use a variety of approaches that should lead to the discovery of 10 or more PAMPsB) Identification of new PRRs in Arabidopsis and selected crops, e.g. barley, wheat, grapevine, brassica (P1, P2, P4 and AP1). We expect that between 5 and 10 PRRs could be identified, or genetically defined within the 3-year periodC) Transformation of Arabidopsis PRRs into crops and evaluation of the resistance phenotype (P1, P2 and AP2). We expect to transform up to 5 PRRs into barley, wheat and brassica and will test them with at least 2 pathogens which infect each crop species.D) Evaluation of developmental and environmental influences on basal resistance responses in grapevine and a diverse range of cereal and brassica accessions (P2, AP1 and AP2).