Deciphering the mechanism of action of the Phytophthora infestans effector Pi13628 in the suppression of MAMP-dependent signalling in tomato

破译致病疫霉效应子 Pi13628 在抑制番茄 MAMP 依赖性信号传导中的作用机制

• 批准号: 162141216
• 负责人: Dr. Frédéric Brunner
• 金额: --
• 依托单位: Centre for Plant Biotechnology and Genomics (CBGP)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/162141216?language=en
• 关键词: Deciphering; mechanism; action; Phytophthora; infestans

Oomycetes are deep-branching eukaryotes that include Hyaloperonospora arabidopsidis, the causal agent of downy mildew on Arabidopsis thaliana, or Phytophthora infestans which causes potato and tomato late blight. In silico analysis predicted that the genome of several oomycete species encodes for a large number of so-called RXLR-(EER) effectors that are assumed to manipulate host cellular functions as part of their infection strategy. The main objective of this project will consist to use a cell-based system in Arabidopsis and tomato to identify and characterize RXLR-(EER) effectors from H.arabidopsidis and P. infestans that 1) suppress plant immune responses triggered by the recognition of invariant microbial structures, termed Pathogen-Associated Molecular Patterns (PAMPs), and/or 2) interfere with the vesicle trafficking system in plant cells. Subsequently, complementary approaches including biochemical and physiological assays will be performed to ascribe functions to the candidate RXLR-(EER) effectors as well as characterizing/discovering their plant targets. A major advantage of the system is that it simplifies the complex pathogen-plant interactions to pure individual signals and synchronized cell-autonomous responses, making it as a powerful high-medium throughput tool to test hundreds of effectors.

卵菌是深分枝真核生物，包括拟南芥霜霉病致病菌拟南芥，或引起马铃薯和番茄晚疫病的致病疫霉。计算机分析预测，几种卵菌物种的基因组编码大量所谓的 RXLR-(EER) 效应器，这些效应器被认为可以操纵宿主细胞功能，作为其感染策略的一部分。该项目的主要目标包括在拟南芥和番茄中使用基于细胞的系统来识别和表征来自拟南芥和致病疫霉的 RXLR-(EER) 效应子，这些效应子 1) 抑制由识别不变微生物结构触发的植物免疫反应，称为病原体相关分子模式 (PAMP)，和/或 2) 干扰植物中的囊泡运输系统 细胞。随后，将进行包括生化和生理测定在内的补充方法，以将功能归因于候选 RXLR-(EER) 效应器，并表征/发现其植物靶标。该系统的一个主要优点是，它将复杂的病原体与植物的相互作用简化为纯粹的个体信号和同步的细胞自主反应，使其成为测试数百个效应器的强大的中高通量工具。