Dinuclear Metal Complexes as DNA-Protein Cross-Linking Agents

该奖项授予无机化学、生物无机化学和金属有机化学计划(MPS/CHE/IBO)，以支持弗吉尼亚联邦大学Nicholas P.Farrell教授通过使用金属络合物来确定DNA-蛋白质相互作用的实验室研究。这项研究涉及使用铂-铂和铂-Ru的系留双核络合物将DNA与蛋白质交联。Pt端倾向于与DNA嘌呤的N7位结合，而Ru端也倾向于与蛋白质中的组氨酰咪唑配位。一些将要使用的铂-铂复合体将DNA锁定为Z型，这应该使它们在将蛋白质与Z-DNA片段特异性地交联方面是独一无二的。两端都有金属离子的过渡金属络合物将被用来将DNA连接到蛋白质上，以努力确定蛋白质如何与DNA相互作用和结合，并探索这些试剂的商业可能性。

This award in the Inorganic, Bioinorganic and Organometallic Chemistry Program and (MPS/CHE/IBO) supports research in the laboratory of Professor Nicholas P. Farrell at Virginia Commonwealth University to determine DNA-protein interactions through the use of metal complexes. The research involves using tethered dinuclear complexes of Pt-Pt and Pt-Ru to crosslink DNA to proteins. The Pt end prefers to bind to the N7 site of DNA purines while the Ru end also shows a preference for coordinating to histidyl imidazoles of proteins. Some of the Pt-Pt complexes to be used lock DNA into the Z form, which should make them unique in specifically crosslinking proteins to sections of Z-DNA. Transition metal complexes with metal ions at either end will be used to attach DNA to proteins in an effort to determine how proteins interact and bind to DNA and to explore the commercial possibilities of these agents.