Probing membrane-peptide and membrane-protein interactions by high-field pulsed EPR spectroscopy (A02)

通过高场脉冲 EPR 光谱探测膜-肽和膜-蛋白质相互作用 (A02)

• 批准号: 107335251
• 金额: --
• 依托单位: Max-Planck-Institut für biophysikalische Chemie (Karl-Friedrich-Bonhoeffer-Institut) (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/107335251?language=en
• 关键词: Probing; membrane; peptide; protein; interactions

With the successful establishment of a protocol to measure inter spin distances in peptides embedded in lipid membranes by means of pulsed EPR, our future goals will be to detect key parameters related to adaptation of model peptides, such as WALP and ¿-peptides, in various lipid environments with specific emphasis on the hydrophobic mismatch. Distance measurements in aligned membranes will reveal the tilt angle of peptide helices. Moreover, experiments at EPR high fields and frequency will give unique insight into the helix periodicity in peptides with different secondary structures.

随着一个协议的成功建立，以测量嵌入在脂质膜中的肽通过脉冲EPR的自旋间距，我们未来的目标将是检测相关的模型肽，如WALP和<$-肽，在各种脂质环境中的适应的关键参数，特别强调的疏水性错配。对齐膜中的距离测量将揭示肽螺旋的倾斜角。此外，EPR高场和高频率的实验将对具有不同二级结构的肽的螺旋周期性提供独特的见解。