CISE Research Instrumentation: Modeling and Image Analysis of Biofilm Systems

汉密尔顿，马丁A.哈金，加里J蒙大拿州立大学-博兹曼CISE研究仪器：生物膜系统的建模和图像分析仪器赠款将支持一个高性能的工作站要求用于生物膜过程的建模和图像分析。 生物膜是一种凝胶状物质，由微生物和它们分泌的粘液组成，附着在基质上。许多涉及液体的自然和工程系统都受到生物膜的深刻影响。生物膜的观察主要是通过复杂的显微镜和表面科学仪器的数字化图像。 重要的是量化的图像和生物膜过程建模。 所要求的工作站必须支持三个相互关联的复杂计算项目： 分析生物膜图像，模拟生物膜中的输运现象，并构建生物膜发展的三维元胞自动机模型。需要定量图像分析来估计基本生物学和物理化学机制的参数。 许多生物膜含有通道，在细胞簇周围提供基本的循环系统。 生物膜中营养物浓度分布和相关微生物生长的任何现实模型都必须考虑通道中的平流运输以及扩散。 需要一个三维模型来表示关键过程，如微生物之间的相互作用，微生物与环境的相互作用，以及微生物附着和脱离生物膜。 元胞自动机建模是构建合理三维模型的最可行的初始策略。

CDA-96-17579 Hamilton, Martin A..Harkin, Gary J. Montana State University - Bozeman CISE Research Instrumentation: Modeling and Image Analysis of Biofilm Systems The instrumentation grant will support a high-powered workstation requested for use in modeling and image analyses of biofilm processes. A biofilm is a gelatinous mass, consisting of microbes and the slime they secrete, attached to a substratum. Many natural and engineered systems involving liquids are profoundly influenced by biofilms. Observations of biofilms are mainly via digitized images from sophisticated microscopes and surface science instruments. It is important to quantify the images and to model biofilm processes. The requested workstation is necessary to support three interrelated, complex computational projects: analyzing biofilm images, simulating transport phenomena in biofilms, and constructing a 3-dimensional cellular automata model of biofilm development. Quantitative image analysis is required to estimate parameters for fundamental biological and physico- chemical mechanisms. Many biofilms contain channels that provide a rudimentary circulatory system around cell clusters. Any realistic model of nutrient concentration profiles and associated microbial growth in the biofilm must account for advective transport in the channels, as well as diffusion. A 3-dimensional model is required to represent key processes such as interactions among microbes, interactions of microbes with the environment, and microbial attachment to and detachment from the biofilm. Cellular automata modeling is the most feasible initial strategy for constructing a plausible 3-dimensional model.