RIMI: Enhancement of Computer Applications in Biological Research

RIMI：增强计算机在生物研究中的应用

• 批准号: 9628640
• 负责人: Scott Williams
• 金额: $ 22.86万
• 依托单位: Meharry Medical College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9628640&HistoricalAwards=false
• 关键词: RIMI; Enhancement; Computer; Applications; Biological

HRD 9628730 New Mexico State University -- Bernard J. McNamara The proposed research will (1) investigate the long term behavior and energetics of the low mass X- ray binary (LMXB) Sco X-1 (2) recruit highly qualified minority undergraduates into the space sciences (3) involve minority undergraduate science and engineering students in two NASA programs and (4) recruit two undergraduate minority students into the astronomy program at NMSU . The project will use X-ray data obtained by the Compton Gamma- Ray Observatory and the X-ray Timing Explorer as well as optical data obtained at Cerro Tololo InterAmerican Observatory and New Mexico State University. The primary research goal is to obtain a multi-year record of the high energy flux and x-ray color of the LMXB Sco X-1 to search for expected long term emission variations from this system. The high energy data sets will also be combined with optical data to better understand this system s energetics. Abstract HRD -9628568 University of Texas at El Paso ---E.Walsh This RIMI project will attempt to provide a cladistic phylogeny of the major planktonic families using a molecular approach. The goals of the proposed research are to (1) isolate, amplify and sequence nuclear ribosomal DNA from representatives of 13 families of the monogonont rotifers, (2) determine the sister group of the monogononts by obtaining comparable data from four bdelloids and a seisonid, (3) construct cladograms and develop phylogenetic hypotheses using molecular characters (4) compare these results with tree topologies derived from morphological characters and (5) evaluate the placement of the Rotifera within the ex isting metazoan phylogenies based on 18S rDNA sequences. The phylogenetic hypotheses generated by the proposed research can be used to further resolve relationships among rortifers. They will constitute a basis from which to incorporate a historical component into understanding questions of broader evolutionary significance. In addition, the relationships among rotifers and other pseudo coelomate taxa will be explored. ABSTRACT HRD- 9628726 City College of C.U.N.Y. ----Moore The long term objective of this laboratory research project is to determine mechanisms cells use to maintain normal DNA function. Mutants will be studied to help determine these requirements. The RIMI project will focus on the characterizations mutants isolated in the laboratory on the basis of their hypersensitivities to killing by ionizing radiation, H2O2 , and a radiomimetic family of chemical congeners. This approach will acquire knowledge from interrelated studies at the cellular and molecular levels, using genetic characterizations, molecular biology and biochemistry, DNA sequence analyses, methodologies for studying DNA damage and repair / transmission and scanning electron microscopy. Cloning and characterizing the blm mutants will enable their relationship to already characterized genes to be determined and seems likely to add new knowledge of general importance to fields of DNA repair , metabolism and genetics. A variety of research training experiences will be provided for students at all levels of education including postdoctoral, graduate, undergraduate, and high school. ABSTRACT HRD---9628640 Meharry Medical College---S.Williams This RIMI project will support the establishment of a core facility in computational molecular biology consisting of state of the art equipment and software ( GCG Package and Molecular Simulations Protein Modeling Programs ), and provide training in their use. This facility will be available to all Meharry researchers. Specific research projects critically dependent on nucleic acid and protein analyses to be directly supported by this initiative are (1) identification of motifs that contribute to structure and function of hetero chromatin; (2) analysis of primary structure and folding patterns of RNA and its effects on viral recombination and replication; and (3) analysis of structural effects of site- directed mutations on flavin-containing monooxygenase. ABSTRACT HRD---9628388 Howard University ---M. McKenna Support is sought for the acquisition and installation of controlled environment growth chambers in the biology department greenhouse. The addition of these chambers will significantly increase research capabilities in plant science, aid in recruitment of talented undergraduates , graduate students and new faculty, and provide a facility that can support precollege outreach programs designed to increase the entry of minority students in science careers. The installation of controlled environment chambers will extend and enhance ongoing research in the areas of plant anatomy and development, phytoremediation, plant ecophysiology, plant ecology , plant systematics, mycology, plant molecular biology and genetics . The facility will allow the institution to maintain experimental plant populations over long periods, maintain clones or plant families for genetic experiments , grow source material for cellular and molecular experiments including tissue culture, DNA analysis, and phytoremediation, maintain plants for systematic study , and design experiments under controlled environmental conditions to study cellular, physiological or ecological processes. ABSTRACT HRD ----9628514 University of Texas at San Antonio----D.Jaffe The primary goal of this RIMI project is to expand the research capabilities of researchers at UTSA with a state of the art computer facility that will satisfy specific needs in molecular sequence analysis and three dimensional image processing. Studies that utilize molecular biological methods regularly produce data that need to be analyzed by comparison against large databases of existing sequences for similarities. pattern searching algorithms are used to unveil nonrandom sequence features that may be of biological relevance. Two computer workstations will be used to carry out molecular sequence analysis using the Intelligenetics Suite, a comprehensive molecular biology software package providing access to sequence databases worldwide and a broad spectrum of sequence analysis tools. The second half of the Computational Biology Facility will focus on cellular anatomy and physiology. Characterization of cellular morphology is critical, for understanding physiological function. Confocal microscopy permits the examination of cellular morphology in three dimensions. In addition

新墨西哥州立大学- Bernard J. McNamara提议的研究将(1)调查低质量X射线双星（LMXB） Sco X-1的长期行为和能量学(2)招募高素质的少数民族本科生进入空间科学(3)在NASA的两个项目中招募少数民族本科科学和工程学生(4)招募两名少数民族本科生进入NMSU的天文学项目。该项目将使用康普顿伽马射线天文台和x射线定时探测器获得的x射线数据，以及Cerro Tololo美洲天文台和新墨西哥州立大学获得的光学数据。主要的研究目标是获得LMXB Sco X-1的高能量通量和x射线颜色的多年记录，以寻找该系统预期的长期发射变化。高能数据集还将与光学数据相结合，以更好地了解该系统的能量学。德克萨斯大学埃尔帕索分校；这个RIMI项目将尝试用分子方法提供主要浮游生物家族的枝状系统发育。本研究的目标是：(1)从13个单轮虫家族的代表中分离、扩增和测序核糖体DNA，(2)通过从4个蛭形虫和1个地震虫中获得可比数据来确定单轮虫的姐妹类群。(3)利用分子特征构建枝形图并提出系统发育假说；(4)将这些结果与形态学特征得出的树拓扑结构进行比较；(5)基于18S rDNA序列评估轮虫属在现有后生动物系统发育中的位置。提出的研究产生的系统发育假设可以用来进一步解决rortifer之间的关系。它们将构成一个基础，在这个基础上，我们将把历史成分纳入对具有更广泛进化意义的问题的理解中。此外，本文还将探讨轮虫与其他拟鞘翅类群之间的关系。这个实验室研究项目的长期目标是确定细胞用来维持正常DNA功能的机制。将对突变体进行研究，以帮助确定这些要求。RIMI项目将重点关注在实验室中分离出的突变体的特征，这些突变体对电离辐射、H2O2和拟辐射化学同系物家族的杀伤具有高度敏感性。这种方法将从细胞和分子水平的相关研究中获取知识，使用遗传特征，分子生物学和生物化学，DNA序列分析，研究DNA损伤和修复/传输和扫描电子显微镜的方法。克隆和鉴定blm突变体将使它们与已经鉴定的基因之间的关系得以确定，而且似乎有可能为DNA修复、代谢和遗传学领域增添具有普遍重要性的新知识。将为包括博士后、研究生、本科生和高中在内的各级教育学生提供各种研究培训经验。摘要HRD- 9628640梅哈里医学院-S。这个RIMI项目将支持建立计算分子生物学的核心设施，包括最先进的设备和软件（GCG包和分子模拟蛋白质建模程序），并提供使用培训。该设施将对所有Meharry研究人员开放。该计划直接支持的主要依赖于核酸和蛋白质分析的具体研究项目有：(1)鉴定与异染色质结构和功能有关的基序；(2)分析RNA的初级结构和折叠模式及其对病毒重组和复制的影响；(3)位点突变对含黄素单加氧酶的结构影响分析。HRD- 9628388霍华德大学硕士。麦肯纳：在生物系的温室中寻求获得和安装受控环境生长室的支持。这些实验室的增加将大大提高植物科学的研究能力，有助于招募有才华的本科生、研究生和新教员，并提供一个设施，可以支持旨在增加少数民族学生进入科学事业的大学前拓展项目。受控环境室的安装将扩展和加强在植物解剖学和发育、植物修复、植物生态生理学、植物生态学、植物系统学、真菌学、植物分子生物学和遗传学等领域正在进行的研究。该设施将允许该机构长期维持实验植物种群，维持克隆或植物家族进行遗传实验，为细胞和分子实验（包括组织培养，DNA分析和植物修复）培养源材料，维护植物进行系统研究，并在受控环境条件下设计实验以研究细胞，生理或生态过程HRD ----9628514德克萨斯大学圣安东尼奥分校----D。这个RIMI项目的主要目标是通过最先进的计算机设备来扩展UTSA研究人员的研究能力，以满足分子序列分析和三维图像处理的特定需求。利用分子生物学方法的研究经常产生数据，需要通过与现有序列的大型数据库进行比较来分析相似性。模式搜索算法用于揭示可能具有生物学相关性的非随机序列特征。两个计算机工作站将使用Intelligenetics Suite进行分子序列分析，Intelligenetics Suite是一个全面的分子生物学软件包，提供访问全球序列数据库和广泛的序列分析工具。计算生物学设施的后半部分将侧重于细胞解剖学和生理学。细胞形态的表征对于理解生理功能是至关重要的。共聚焦显微镜允许在三维检查细胞形态。除了