Research Planning Grant: Comparative Actions of Somato- lactin, Prolactin, and Growth Hormone on Reproductive Function in Fundulus heteroclitus

研究计划资助：生长激素、催乳素和生长激素对异斜眼生殖功能的比较作用

• 批准号: 9629665
• 负责人: Susan Safford
• 金额: $ 1.79万
• 依托单位: Lincoln University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 1997-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9629665&HistoricalAwards=false
• 关键词: Research; Planning; Grant; Comparative; Actions

Results from several studies suggest that somatolactin (SL), prolactin (PRL), and growth hormone (GH), structurally related proteins, are involved in some aspects of sexual maturation in fishes. To investigate the physiological roles of SL, a model fish species is needed which has been extensively studied and can provide numerous animals and eggs for study. The mummichog, Fundulus heteroclitus, is such a species, and Dr. Safford's preliminary research is designed to begin to develop it as a model for comparing the actions of SL, PRL, and GH. The objectives of the preliminary project are (1) to determine if proteins of the appropriate molecular weights from F. heteroclitus pituitary extracts bind to antibodies against SL, PRL, and GH; (2) to determine if these immunoreactive proteins are found in the appropriate areas within the F. heteroclitus pituitary, and (3) to determine if the F. heteroclitus genome has gene sequences corresponding to conserved regions of fish SL. The experiments for the follow-up project will focus on comparative actions of SL, PRL, and GH on reproductive function in F. heteroclitus, as more studies on SL focus on reproduction than on other processes and there is evidence from the literature that PRL and GH also have reproductive functions. If these three hormones are shown to have some functions in F. heteroclitus, then SL, PRL, and GH will be cloned so that recombinant F. heteroclitus hormones.will be available for more detailed studies in a homologous system.

一些研究结果表明，生长激素（SL），催乳素（PRL）和生长激素（GH），结构相关的蛋白质，参与鱼类性成熟的某些方面。 为了研究SL的生理作用，需要一种已被广泛研究并可以提供大量动物和卵用于研究的模式鱼类。 木乃伊猪，底heteroclitus，就是这样一个物种，和萨福德博士的初步研究旨在开始发展它作为一个模型，比较SL，PRL和GH的行动。 初步研究的目标是：（1）确定是否从F.（2）确定这些免疫反应蛋白是否存在于F. heteroclitus pituitary;（3）确定F. heteroclitus基因组具有与鱼类SL的保守区域相对应的基因序列。 后续的实验将着重于SL、PRL和GH对F.因此，对SL的研究更多地集中在生殖而不是其他过程上，并且文献中有证据表明PRL和GH也具有生殖功能。 如果这三种激素在F. heteroclitus，然后SL，PRL和GH将被克隆，以便重组F。将在同源系统中进行更详细的研究。