E. coli Termination Factor Rho, pBR322 Rop, and mRNA Degradation

9612367 Gronostajski核因子I（NFI）是一类在遗传学上保守的位点特异性DNA结合蛋白，其在包括生物体发育在内的基本细胞过程中发挥重要作用。这些研究检查了含有单个NFI基因的生物体中的简单发育系统。NFI在C.将通过使用Tcl靶向诱变在NFI基因中产生缺失突变体来研究线虫。纯合无效突变体的表型，以及表达特定突变形式的NFI的生物体的表型将产生重要的见解，了解这个进化上保守的DNA结合蛋白家族在C.线虫和其他生物。将分析该缺失突变体的细胞活力、胚胎和胚胎后发育表型。如果无效等位基因产生早期致死表型，则其将在含有野生型基因的染色体外阵列的菌株中携带，这将允许通过阵列的分配产生纯合缺失动物。这种“反向遗传”的方法来理解NFI在C。elegans的发展也将允许测试脊椎动物NFI基因是否可以补充C。elegans基因，从而评估C. elegans突变体可能在分析其他多基因家族参与脊椎动物的发展。 ***

9612367 Gronostajski Nuclear Factor I (NFI) is one of a class of phylogenetically-conserved site-specific DNA-binding proteins that play an essential role in fundamental cellular processes including organismal development. These studies examine a simple developmental system in a organism containing a single NFI gene. The function of the NFI in the growth and development of C. elegans will be studied by the generation of deletion mutants in the NFI gene using Tcl targeted mutagenesis. The phenotype of homozygous null-mutants, and of organisms expressing specific mutated forms of NFI will yield important insights into how this family of evolutionarily-conserved DNA-binding proteins function in C. elegans and other organisms. The phenotype of this deletion mutant will be analyzed in respect to cell viability, embryonic, and post-embryonic development. If the null-allele generates an early lethal phenotype, it will be carried in a strain containing an extrachromosomal array of the wild-type gene which will allow the generation of homozygous deletion animals by partitioning of the array. This "reverse genetic" approach to understanding NFI function during C. elegans development will also allow testing whether the vertebrate NFI genes can complement the C. elegans gene and thus assess how useful C. elegans mutants may be in the analysis of other multigene families involved in vertebrate development. ***