E. coli Termination Factor Rho, pBR322 Rop, and mRNA Degradation

大肠杆菌终止因子 Rho、pBR322 Rop 和 mRNA 降解

• 批准号: 9630780
• 负责人: Barbara Stitt
• 金额: $ 25.5万
• 依托单位: Temple University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630780&HistoricalAwards=false
• 关键词: E.; coli; Termination; Factor; Rho

9612367 Gronostajski Nuclear Factor I (NFI) is one of a class of phylogenetically-conserved site-specific DNA-binding proteins that play an essential role in fundamental cellular processes including organismal development. These studies examine a simple developmental system in a organism containing a single NFI gene. The function of the NFI in the growth and development of C. elegans will be studied by the generation of deletion mutants in the NFI gene using Tcl targeted mutagenesis. The phenotype of homozygous null-mutants, and of organisms expressing specific mutated forms of NFI will yield important insights into how this family of evolutionarily-conserved DNA-binding proteins function in C. elegans and other organisms. The phenotype of this deletion mutant will be analyzed in respect to cell viability, embryonic, and post-embryonic development. If the null-allele generates an early lethal phenotype, it will be carried in a strain containing an extrachromosomal array of the wild-type gene which will allow the generation of homozygous deletion animals by partitioning of the array. This "reverse genetic" approach to understanding NFI function during C. elegans development will also allow testing whether the vertebrate NFI genes can complement the C. elegans gene and thus assess how useful C. elegans mutants may be in the analysis of other multigene families involved in vertebrate development. ***

9612367 Gronostajski 核因子 I (NFI) 是一类系统发育保守的位点特异性 DNA 结合蛋白之一，在包括有机体发育在内的基本细胞过程中发挥重要作用。这些研究检查了含有单个 NFI 基因的生物体中的简单发育系统。将通过使用 Tcl 靶向诱变在 NFI 基因中产生缺失突变体来研究 NFI 在秀丽隐杆线虫生长和发育中的功能。纯合无效突变体以及表达 NFI 特定突变形式的生物体的表型将为了解这一进化保守的 DNA 结合蛋白家族如何在秀丽隐杆线虫和其他生物体中发挥作用提供重要的见解。将在细胞活力、胚胎和胚胎后发育方面分析该缺失突变体的表型。如果无效等位基因产生早期致死表型，则它将携带在含有野生型基因的染色体外阵列的菌株中，这将允许通过阵列的分区产生纯合缺失动物。这种了解线虫发育过程中 NFI 功能的“反向遗传”方法也将允许测试脊椎动物 NFI 基因是否可以补充线虫基因，从而评估线虫突变体在分析参与脊椎动物发育的其他多基因家族中的有用性。 ***