Mechanism of Nonhomologous RNA Recombination in Brome MosaicVirus: Separating Replication and Recombination

雀麦花叶病毒非同源 RNA 重组机制：复制与重组分离

• 批准号: 9630794
• 负责人: Jozef Bujarski
• 金额: $ 32.7万
• 依托单位: Northern Illinois University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630794&HistoricalAwards=false
• 关键词: Mechanism; Nonhomologous; RNA; Recombination; Brome

9630794 Bujarski The molecular mechanisms of RNA recombination in RNA viruses using brome mosaic virus(BMV) as a model system will be investigated. BMV mutants isolated and characterized during the tenure of the previous award has demonstrated that replication and recombination functions can be unlinked. As an approach to understanding how BMV proteins participate in nonhomologous heteroduplex-mediated recombination, we will 1. analyze the involvement of the 1a helicase domain in recombination, 2. analyze the involvement of RNA polymerase (2a protein) core domain in recombination and 3. study the role of the N-domain of the 2a protein in recombination. %%% Only a limited amount of information concerning the mechanisms involved in non-homologous RNA-RNA recombination is available. Such recombination plays a key role in RNA virus genome plasticity, as it can lead to the generation of new virus strains and species. An understanding of the basic mechanisms of RNA-RNA recombination will provide valuable insight into viral evolution and diversity and could provide important practical information which could be used for applied goals. ***

9630794 Bujarski以雀麦花叶病毒为模型系统，研究核糖核酸病毒中核糖核酸重组的分子机制。在前一个奖项的任期内分离和鉴定的BMV突变体已经证明，复制和重组功能可以分离。为了了解BMV蛋白如何参与非同源异源双链介导的重组，我们将1.分析1a解旋酶结构域在重组中的参与，2.分析RNA聚合酶(2a蛋白)核心区在重组中的参与，3.研究2a蛋白的N-结构域在重组中的作用。关于非同源RNA-RNA重组所涉及的机制，只有有限的信息可用。这种重组在RNA病毒基因组的可塑性中起着关键作用，因为它可以导致新病毒株和新物种的产生。了解RNA-RNA重组的基本机制将为病毒的进化和多样性提供有价值的见解，并可能提供重要的实用信息，可用于应用目标。***