RUI: Studies on Intermolecular Interactions Nuclear Transport of SV40 Vp1

小行星9630904 猴病毒40（SV40）是一种小的DNA病毒，在猴细胞中经历一个允许的感染过程。 其主要结构蛋白Vp1是其五聚体壳粒的基本单位。 在允许感染的后期，它在感染细胞的细胞质中合成，并沿着次要结构蛋白被转运到细胞核，在那里进行病毒组装。 SV40组装的早期步骤，包括Vp1的早期寡聚化和与次要结构蛋白的相互作用，以及其核转运的机制，尚未完全探索。 几条线的证据表明，在细胞质中的相互作用Vp1复合物的形成之前，核运输。 有证据表明，二硫键可能参与Vp1寡聚体的稳定形成，寡聚化可能在Vp1的核转运中发挥作用。 在这里，我们提出了研究早期Vp1相互作用复合物在允许的哺乳动物细胞系，包括确定特定的半胱氨酸，可能参与二硫键连接Vp1寡聚化。 寡聚化和复合物形成的潜在作用与小的结构蛋白在核运输的Vp1也将被探讨。 ***

9630904 Gharakhanian Simian Virus 40 (SV40) is a small DNA virus which undergoes a permissive course of infection in simian cells. Its major structural protein, Vp1, is the basic unit of its pentameric capsomeres. It is synthesized in the cytoplasm of the infected cell late in the permissive infection, and along with the minor structural proteins is transported to the nucleus where viral assembly takes place. The early steps of SV40 assembly, including the early oligomerization of Vp1 and interactions with the minor structural proteins, and the mechanism of its nuclear transport, have not been fully explored. Several lines of evidence suggest the formation of interactive Vp1 complexes in the cytoplasm prior to nuclear transport. Evidence suggests that disulfide bonds may be involved in the stable formation of Vp1 oligomers and that oligomerization may play a role in the nuclear transport of Vp1. Here, we propose studies on the early Vp1 interactive complexes in permissive mammalian cell lines including identifying specific cysteines that may be involved in the disulfide bonded Vp1 oligomerization. The potential role of oligomerization and complex formation with the minor structural proteins in the nuclear transport of Vp1 will also be explored. ***