RUI: Studies on the Pentamerization of SV40 VPL In Vitro

关于病毒衣壳蛋白自组装的研究， 作为分子间相互作用的模型系统。 的 猴病毒40（SV40）的主要衣壳蛋白是在 感染细胞的细胞质并被转运到细胞核 用于病毒装配。 蛋白质形成了 SV40衣壳的五聚体衣壳。 Vp1五聚体是 在感染细胞的细胞质和体外均可检测到。 Vp1壳粒形成所涉及的相互作用的性质， 以及所涉及的残留物都是未知的。 胞质Vp1 五聚体的形成不仅是SV40组装的初始阶段， 但也可能在Vp 1的有效核转运中起作用。 在这里，我们建议检查化学性质和氨基酸 参与Vp1多聚体形成的残基。 结果 建议的实验将用于研究的作用， 五聚体的形成在运输的Vp 1的核。 %%% 由于其基因结构相对简单， 40已被用作研究病毒装配的模型系统， 结构和功能。 病毒在细胞核中聚集， 因此，它也是一个理想的模型系统， 核定位和核定位信号。 这 项目将利用SV40的这些功能， 我们对病毒装配和核质 贩卖人口 此外，该项目将提供一个机会， 也是本科生参与生物学的一个载体 research.

Studies on the self assembly of viral capsid proteins have served as a model system for intermolecular interactions. The major capsid protein of simian virus 40 (SV40) is synthesized in the cytoplasm of infected cells and is transported to the nucleus shortly after synthesis for viral assembly. The protein forms the pentameric capsomeres of the SV40 capsid. Vp1 pentamers are detectable both in the cytoplasm of infected cells and in vitro. The nature of the interactions involved in Vp1 capsomere formation, as well as the residues involved, are not known. Cytoplasmic Vp1 pentamer formation is not only the initial stage of SV40 assembly, but may also have a role in the efficient nuclear transport of Vp1. Here, we propose to examine the chemical nature and the amino acid residues involved in Vp1 multimer formation in vitro. Results from the proposed experiments will later be used to study the role of pentamer formation in the transport of Vp1 to the nucleus. %%% Due to the relative simplicity of its gene structure, simian virus 40 has been used as a model system for studies of virus assembly, structure, and function. The virus assembles in the nucleus of the host cell, and thus it is also an ideal model system for study of nuclear localization and nuclear localization signals. This project will take advantage of these features of SV40 to increase our understanding of viral assembly and nucleocytoplasmic trafficking. In addition, the project will provide an opportunity and a vehicle for undergraduate student involvement in biological research.