Dissertation Research: Radiation, Evolution, and Interaction of Two Sulawesi Macaque Species

论文研究：两种苏拉威西猕猴的辐射、进化和相互作用

• 批准号: 9631767
• 负责人: Don Melnick
• 金额: $ 1.14万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9631767&HistoricalAwards=false
• 关键词: Dissertation; Research; Radiation; Evolution; Interaction

Speciation, a process whereby a new evolutionarily independent entity arises, underlies much of the biological diversity seen today. For this reason, it has been the focus of much theoretical work leading to many models of how new species arise and many, often controversial definitions of what a species is. Despite this work, few empirical studies of primates have shed light on the dynamics of speciation. The researchers propose to examine in detail two putative species of cercopithecine monkey and their hybrids, in order to gain a clearer understanding of the genetic consequences of geographic separation and subsequent intergradation. The two species are members of the macaque complex, Macaca maurus and M. tonkeana, on the Indonesian island of Sulawesi. Genetic material will be collected from several hundred individuals within the range of each species and across the intervening hybrid zone. Using automated DNA sequencing technology, the mitochondrial and nuclear genomes will be surveyed for informative markers that reveal the direction and rate of gene flow across the hybrid zone, the relative contribution of males and females of each species to this hybridization process, and the genetic effects of hybridization on the long term evolutionary integrity of each species. Female contributions will be tracked using mtDNA markers, while male mediated gene flow will be reconstructed using nDNA microsatellites. This study will shed light on the process of species formation among higher primates, factors leading to its subsequent breakdown, and possibly why there are so many species of macaque monkeys on Sulawesi.

物种形成是一个新的进化上独立的实体产生的过程，是今天所看到的生物多样性的基础。由于这个原因，它一直是许多理论工作的焦点，导致了许多关于新物种如何出现的模型和许多关于物种是什么的定义，这些定义往往是有争议的。 尽管有这些工作，但很少有灵长类动物的实证研究揭示了物种形成的动力学。 研究人员建议详细研究两种假定的猕猴及其杂交种，以便更清楚地了解地理分离和随后的过渡的遗传后果。 这两个物种是猕猴复合体的成员，猕猴maurus和M。印度尼西亚苏拉威西岛上的一种名为tonkeana的植物。 遗传物质将从每个物种范围内的数百个个体中收集，并跨越中间的杂交区。 使用自动化DNA测序技术，线粒体和核基因组将调查信息标记，揭示方向和速度的基因流在杂交区，相对贡献的男性和女性的每个物种的杂交过程中，和杂交的遗传效应对每个物种的长期进化的完整性。女性的贡献将使用mtDNA标记进行跟踪，而男性介导的基因流将使用nDNA微卫星进行重建。 这项研究将揭示高等灵长类动物物种形成的过程，导致其随后崩溃的因素，以及可能为什么苏拉威西岛上有这么多种类的猕猴。