RUI: Structural Analysis of Paramutation at the R Locus of Zea Mays

RUI：玉米 R 基因座副突变的结构分析

• 批准号: 9631967
• 负责人: Elsbeth Walker
• 金额: $ 30万
• 依托单位: Mount Holyoke College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9631967&HistoricalAwards=false
• 关键词: RUI; Structural; Analysis; Paramutation; Locus

9631967 Walker This work will involve the molecular cloning and characterization of the R-mb complex of maize. The organization of the complex as a whole will be determined through the production of derivatives of the R-mb complex that have lost one or more genes through unequal crossing over. The number, identity and expression of each individual gene of the complex will be determined. The specific regions of the complex involved in paramutagenicity will be determined and a more limited structural characterization of a diverse collection of R geographic alleles will be made. Undergraduate students will be introduced to basic biological research by working on the described projects. %%% Epigenetic inheritance, nuclear inheritance that is not based on DNA sequence, has been recognized in all of the eukaryotic kingdoms. To approach the question of why certain genes are subject to epigenetic regulation while others are not, the structures/sequences of epigenetically regulated genes must be determined. The R paramutation system of corn is ideal for an analysis of this phenomenon because the important components have been localized to the R locus itself and there are a diverse set of alleles with different phenotypes available. Detailed structural analysis of the paramutable R-r complex suggest that a small region of only about two kilobases out of 200 kb is involved in the paramutation process. ***

小步行者9631967 这项工作将涉及玉米R-mb复合物的分子克隆和表征。复合物的整体结构将通过产生R-mb复合物的衍生物来确定，这些衍生物通过不平等交换失去了一个或多个基因。将确定复合物的每个单独基因的数量、身份和表达。将确定参与副突变的复合体的特定区域，并对R地理等位基因的多样性集合进行更有限的结构表征。 本科生将通过所描述的项目工作介绍基础生物学研究。 表观遗传，即不以DNA序列为基础的核遗传，在所有的真核生物界都得到了承认。为了解决为什么某些基因受到表观遗传调控而其他基因不受表观遗传调控的问题，必须确定表观遗传调控基因的结构/序列。玉米的R副突变系统是分析这一现象的理想系统，因为重要的组分已经定位于R基因座本身，并且存在具有不同表型的不同等位基因组。详细的结构分析的paramutable R-r复合物表明，一个小区域，只有约两个酶出200 kb的参与paramutation过程。 ***