CAREER: New Methods of Creating Artifical Receptors for Proteins

职业：创建蛋白质人工受体的新方法

• 批准号: 9701975
• 负责人: Sanku Mallik
• 金额: $ 8万
• 依托单位: University of North Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9701975&HistoricalAwards=false
• 关键词: CAREER; New; Methods; Creating; Artifical

Professor Mallik's research focuses on the design, synthesis and evaluation of liposome based receptors for protein surfaces. Mixed liposomes derived from metal containing lipids, polymerizable ionic lipids, and polymerizable hydrogen bonding lipids are equilibrated with the template protein carbonic anhydrase to create a three-dimensional, complementary replica of the protein surface. Synthetic targets include lipids containing differing head groups and photopolymerizable diene or diyne tails that, after equilibration, are photochemically polymerized to achieve a semi-rigid, protein-specific receptor. Efficacy of the photopolymerized template synthesis is quantified by measuring the protein-replica binding constants. Educational activities include introducing a graduate bio-organic course and addressing undergraduate needs by establishing a Virtual Organic Chemistry classroom on the Web to facilitate student-teacher communication. With this CAREER award, the Organic Dynamics Program supports the research and educational activities of Professor Sanku Mallik of the Department of Chemistry at the University of North Dakota. Professor Mallik's research is focused on the creation of a three-dimensional protein recognition surface by using a specific protein to direct the formation of a collection of molecules with which it interacts. Subsequent chemical linking of these molecules into a polymer effectively leaves a footprint of the protein in the resulting polymer. In contrast to the considerable synthetic effort involved in other approaches to molecular recognition, this approach is expected to generate simply and rapidly a highly specific binding site for the target protein. Professor Mallik's educational activities include developing a graduate curriculum targeting the interface between organic and biological chemistry and facilitating student-teacher communication through a Virtual Classroom on the Web.

Mallik教授的研究重点是设计、合成和评估基于脂质体的蛋白质表面受体。混合脂质体来源于含有脂质的金属，可聚合离子脂质和可聚合氢键脂质，与模板蛋白碳酸酐酶平衡，以创建蛋白质表面的三维互补复制品。合成靶标包括含有不同头基和可光聚合的二烯或二烯尾部的脂质，这些二烯或二烯尾部在平衡后被光化学聚合以获得半刚性的蛋白质特异性受体。光聚合模板的合成效果是通过测量蛋白质复制体的结合常数来量化的。教育活动包括引入研究生生物有机课程和通过在网络上建立虚拟有机化学教室来满足本科生的需求，以促进学生与教师的交流。有了这个职业奖，有机动力学项目支持北达科他州大学化学系的Sanku Mallik教授的研究和教育活动。Mallik教授的研究重点是通过使用特定的蛋白质来指导与之相互作用的分子集合的形成，从而创建三维蛋白质识别表面。这些分子随后的化学连接形成聚合物，有效地在聚合物中留下了蛋白质的足迹。与其他分子识别方法所涉及的大量合成工作相比，该方法有望简单而快速地为目标蛋白生成高度特异性的结合位点。Mallik教授的教育活动包括开发一个研究生课程，目标是有机化学和生物化学之间的接口，并通过网络上的虚拟教室促进学生与教师的交流。