Japan STA Program: Dissecting the Molecular Mechanisms of the Signal Transduction Pathway Leading to Ethylene Dependent Transcription

日本 STA 项目：剖析导致乙烯依赖性转录的信号转导途径的分子机制

• 批准号: 9703835
• 负责人: Susan Fujimoto
• 金额: $ 0.3万
• 依托单位: Fellowships
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9703835&HistoricalAwards=false
• 关键词: Japan; STA; Program; Dissecting; Molecular

Fujimoto 9703835 This award supports a 24 month Science and Technology Agency of Japan (STA) Postdoctoral Fellowship for Dr. Susan Y. Fujimoto at the National Institute of Bioscience and Human Technology, Tsukuba, Japan. Dr. Fujimoto will work with Dr. Masaru Takagi, Senior Researcher in the Plant Molecular Biology Laboratory, on a research project entitled, "Dissecting the Molecular Mechanisms of the Signal Transduction Pathway Leading to Ethylene Dependent Transcription." The goal of the proposed research is to identify molecular components of the pathway leading from the perception of the plant hormone ethylene to a physiological response. How this gas acts as an important growth regulator in plants is not yet well understood. Elucidation of this pathway would not only offer fundamental insights for basic science but would also provide a significant impact to plant biotechnology. Using the model plant Arabidopsis thaliana, factors that are involved in the transmission of signals necessary to initiate transcription of genes responsive to ethylene will be investigated. Ethylene responsive genes have already been isolated from Arabidopsis and a group of proteins termed AERBs (Arabidopsis Ethylene-Responsive Binding proteins) that interact with these genes have been characterized. In the proposed research, AERB gene expression will be analyzed and compared with expressions in Arabidopsis mutants affected in their response to ethylene. In addition, the possibility of protein modification and identification of factors interacting with the AERBs in response to ethylene will be examined. By using ethylene responsive genes in conjunction with known ethylene response mutants, one can dissect and possibly order the genes comprising this important and complex pathway. ***

该奖项9703835该奖项为日本国家生物科学与人类技术研究所Susan Y. Fujimoto博士的日本科学技术机构（STA）提供了24个月的科学技术署（STA）。 Fujimoto博士将与植物分子生物学实验室的高级研究员Masaru Takagi博士合作，题为“解剖导致乙烯依赖转录的信号转导途径的分子机制”。 拟议的研究的目的是确定从植物激素乙烯感知到生理反应的途径的分子成分。 这种气体如何作为植物中重要的生长调节剂尚不清楚。 阐明这一途径不仅会为基础科学提供基本见解，而且还将为植物生物技术带来重大影响。 使用模型植物拟南芥，将研究涉及启动对乙烯基因转录所需的信号传播的因素。 乙烯反应基因已经从拟南芥和称为AERB的一组蛋白（拟南芥乙烯反应性结合蛋白）中分离出来，与这些基因相互作用已经表征。 在拟议的研究中，将分析AERB基因表达，并将其与拟南芥突变体的表达进行比较。 另外，将检查蛋白质修饰和鉴定与乙烯相互作用的因素与AERB相互作用的可能性。 通过将乙烯响应基因与已知的乙烯反应突变体结合使用，可以剖析并可能订购包含这一重要且复杂途径的基因。 ***