Optimization and Applications of cspA, the Major E.coli Cold-Shock Promoter

大肠杆菌冷休克主要启动子cspA的优化及应用

• 批准号: 9707729
• 负责人: Francois Baneyx
• 金额: $ 28.73万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-15 至 2001-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9707729&HistoricalAwards=false
• 关键词: Optimization; Applications; cspA; Major; E.coli

9707729 Baneyx The purpose of this research is to minimize the improper folding and aggregation of proteins produced in E. coli, hence reducing a very costly part (refolding) of the downstream processing. This proposal follows previous observations that a low-temperature, inducible cold-shock promoter drives the proper folding of recombinant proteins. Unfortunately, after a short period the promoter is repressed at the lower temperature and protein production drops. The two major aims of the research are to eliminate the repressor gene segment believed to lie on the 5' non- coding region of the cold-shock promoter gene and to coexpress protein disulfide isomerases to promote the proper folding and disulfide bonding of the proteins. ***

9707729 Baneyx本研究的目的是最大限度地减少E.大肠杆菌，因此减少了下游加工中非常昂贵的部分（重折叠）。 这个建议遵循以前的观察，低温，诱导型冷休克启动子驱动重组蛋白的正确折叠。 不幸的是，在较低的温度下，启动子在短时间内被抑制，蛋白质产量下降。 该研究的两个主要目的是消除被认为位于冷休克启动子基因的5'非编码区的阻遏基因片段和共表达蛋白质二硫键异构酶以促进蛋白质的正确折叠和二硫键结合。 ***