Hydrodynamic Manipulation of DNA Molecules

摘要：布朗动力学模拟将用于预测长DNA分子在简单流动中的拉伸行为，包括均匀拉伸、均匀、剪切和平面泊泽维尔流。这些模拟的结果将直接与在相应的简单流动中对单个长荧光DNA分子进行的实验进行比较。该研究将考虑长聚合物分子在拉伸或剪切流动中伸展的瞬态过程，并确定描述稳态均匀流动的物理力是否适用于复杂的解开瞬态流动过程。当模拟与实验结果相结合时，也可能有助于解释为什么光散射研究表明在这种流动中聚合物很少拉伸。这些研究不仅应用于聚合物流体力学的传统领域，如湍流减阻和现在的多孔介质，而且还与涉及DNA微操作的技术相关，包括“流体固定”的新技术，即利用流体动力学流动拉伸长DNA分子以帮助进行遗传定位。

CTS-9707778 R.G. LARSON UNIV. OF MICHIGAN SUMMARY Brownian dynamics simulations will be performed to predict the stretching behavior of long DNA molecules in simple flows, including uniform extensional, uniform, shear, and plane Poiseuille flows. The results of these simulations will be compared directly with experiments on single long, fluorescing, molecules of DNA in corresponding simple flows. The research will consider the transient process by which long polymer molecules stretch out in extensional or shear flows, and determine whether or not the physical forces that describe uniform flows at steady state work for the complex unraveling transient flow processes. The simulations, when combined with the experimental results, may also help explain why light scattering studies indicate little polymer stretching in such flows. These studies have applications not only in traditional areas of polymer fluid mechanics, Such as turbulent drag reduction and now through porous media, but are also relevant to Technologies involving DNA micro-manipulation, including the new technology of "fluid fixation", i.e., the use of hydrodynamic flows to stretch long DNA molecules to aid in genetic mapping.