Collaborative Research: (EGB) Multisubstrate Biodegradation Kinetics of Polycyclic Aromatic Hydrocarbons (PAHs) in NAPL-Contaminated Soils

9708406彼得斯本项目旨在研究控制多环芳烃（PAHs）混合物在apl污染土壤中的行为的化学，物理和微生物过程。多环芳烃是一类重要的有机污染物，通常与煤焦油、杂酚油和柴油等多组分有机污染物有关。这些污染物通常以非水相液体（NAPLs）的形式存在。对潜在机制的清晰理解和全面的成分建模方法对于理解这些污染物在更大范围和长时间内的影响至关重要。该项目的具体目标是：(1)深入了解单一和多底物系统中多环芳烃的相对生物降解性和底物相互作用；(2)将NAPL污染系统中的生物降解率与控制生物可利用性的热力学相平衡和溶解动力学过程联系起来；(3)构建nal污染土壤中多环芳烃行为的综合数学模型，并通过使用PAH-NAPL污染的土壤进行实验来验证模型公式。实验研究旨在探索多环芳烃溶解与生物降解的相互关系，并观察在不同初始底物浓度和比例条件下底物相互作用的存在或不存在。一个目的是确定从单一底物实验推断的动力学参数可以与捕获底物相互作用效应的数学公式结合使用的程度，以描述具有多种多环芳烃底物的系统中发生的情况。这项工作的一个新颖方面是使用合成NAPLs作为模型有机液体来模拟多组分污染物，如煤焦油，这允许在实验设计和分析表征中进行控制。

9708406 Peters This project is designed to investigate the chemical, physical, and microbiological processes governing the behavior of mixtures of polycyclic aromatic hydrocarbons (PAHs) in NAPL-contaminated soils. PAHs are an important class of organic pollutants that are commonly associated with multicomponent organic contaminants such as coal tar, creosote, and diesel fuel. These contaminants commonly exist as non-aqueous phase liquids (NAPLs). A clear understanding of underlying mechanisms and a comprehensive compositional modeling approach is essential to understanding the impacts of these contaminants on a larger scale and over a long time periods. The specific objectives of this project are: (I) to gain insights about relative biodegradabilities and substrate interactions of PAHs in sole and multi-substrate systems, (ii) to relate biodegradation rates in NAPL contaminated systems to the thermodynamic phase equilibrium and dissolution kinetic processes governing bioavailability, and (iii) to construct an integrated mathematical model of PAH behavior in NAPL-contaminated soils and to validate model formulation through experimentation using PAH-NAPL-contaminated soils. Experimental studies are designed to explore the interrelation of PAH dissolution and biodegradation, and to observe the presence or absence of substrate interactions under various conditions of initial substrate concentrations and proportions. One aim is to ascertain the extent to which kinetic parameters inferred from sole substrate experiments can be used in conjunction with a mathematical formulation that captures substrate interaction effects to describe what happens in a system with multiple PAH substrates. A novel aspect of this work is the use of synthetic NAPLs as models organic liquids to simulate multicomponent contaminants such as coal tars, which permits control in experimental design and analytical characterization.