Nucleic Acid Films Studied by Surface Plasmon Resonance Spectroscopy

通过表面等离子共振光谱研究核酸膜

• 批准号: 9709347
• 负责人: Rosina Georgiadis
• 金额: $ 5万
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9709347&HistoricalAwards=false
• 关键词: Nucleic; Acid; Films; Studied; Surface

This Career Advancement Award is supported by the Analytical and Surface Chemistry Program. Professor Georgiadis and her students at Boston University will study the interactions and properties of nucleic acids at interfaces using in situ two-color surface plasmon resonance spectroscopy. Self-assembled monolayers of oligonucleotides on surfaces and the factors that control hybridization selectivity in DNA and peptide nucleic acid films will be studied. This work has potential for developing extended DNA arrays that will be useful in studies DNA-drug interactions. Monolayers of biomolecules on surfaces will be studied by an extremely sensitive surface spectroscopic method, surface plasmon resonance spectroscopy, using two colors of light. This new method has been developed in this laboratory. The properties of the biomolecules as they interact with compounds that are candidates for drugs will be measured and used to understand these chemical reactions. This information will also be useful in developing rapid nucleotide sequencing data for DNA and other nucleic acids.

该职业发展奖由分析和表面化学项目支持。 波士顿大学的 Georgiadis 教授和她的学生将利用原位双色表面等离子体共振光谱研究界面处核酸的相互作用和特性。 将研究表面上寡核苷酸的自组装单层以及控制 DNA 和肽核酸膜中杂交选择性的因素。 这项工作有可能开发出扩展的 DNA 阵列，这将有助于研究 DNA-药物相互作用。 表面上的生物分子单层将通过极其灵敏的表面光谱方法（表面等离子体共振光谱）使用两种颜色的光进行研究。 这种新方法是本实验室开发的。 生物分子与候选药物化合物相互作用时的特性将被测量并用于理解这些化学反应。 该信息还可用于开发 DNA 和其他核酸的快速核苷酸测序数据。