SGER: Microsatellite Typing Methods to Free-Ranging Field Populations

PI：Scott Creel #9712613 这项探索性研究（SGER）的小额赠款涉及微卫星DNA方法的开发和应用，用于从野外样本中对非洲野狗进行基因分型。 PI正在进行的研究为开发一套可能具有深远适用性的新技术提供了难得的机会。 本研究中先前获取血液和组织样本的努力对动物造成了伤害，这在濒危物种中是不能容忍的。 从食肉动物的废弃物中提取的DNA的替代分析方法特别困难，因为如果DNA材料降解，其质量会降低。 然而，这项研究中最近开发的新技术已经显示出提供可靠和有效的遗传分析方法的希望。 该项目是一种全新的、完全非侵入性的方法，用于从野外种群中收集DNA。 这一进步可以使许多动物物种的新技术的开发和应用成为可能，这对濒危物种的实地研究和保护特别有价值。

PI: Scott Creel #9712613 This small grant for exploratory research (SGER) concerns the development and application of microsatellite DNA methods for genetic typing of African wild dogs from field samples. The PI's ongoing research has allowed a rare opportunity to develop a new set of techniques that may have far-reaching applicability. Previous efforts in this study to obtain blood and tissue samples have caused injury to the animals, which cannot be tolerated in an endangered species. Alternative means of analysis of DNA extracted from wasted material of carnivores has been especially difficult, as the quality if the DNA material is degraded. However, new techniques recently developed in this study have shown promise in providing reliable and efficient means of genetic analysis from samples collected in the field. The project is a novel, completely non-invasive approach to collection of DNA from field populations. This advanced could enable the development and application of new techniques for many animal species, which would be especially valuable in field research and conservation of endangered species.