SGER: Microsatellite Typing Methods to Free-Ranging Field Populations

点击翻译按钮获取中文摘要

PI: Scott Creel #9712613 This small grant for exploratory research (SGER) concerns the development and application of microsatellite DNA methods for genetic typing of African wild dogs from field samples. The PI's ongoing research has allowed a rare opportunity to develop a new set of techniques that may have far-reaching applicability. Previous efforts in this study to obtain blood and tissue samples have caused injury to the animals, which cannot be tolerated in an endangered species. Alternative means of analysis of DNA extracted from wasted material of carnivores has been especially difficult, as the quality if the DNA material is degraded. However, new techniques recently developed in this study have shown promise in providing reliable and efficient means of genetic analysis from samples collected in the field. The project is a novel, completely non-invasive approach to collection of DNA from field populations. This advanced could enable the development and application of new techniques for many animal species, which would be especially valuable in field research and conservation of endangered species.