Integrated Bioprocessing/Molecular Strategies for Heterologous Protein Production in Perfusion Plant Cell Cultures

9712916 Su该提案试图通过整合三种新技术来改善植物细胞培养中异源蛋白的生产：新的启动子;新的报道基因;和改进的灌注生物反应器。 最近鉴定的强的、可诱导的、酸性磷酸酶启动子和信号序列将与作为报告蛋白的绿色荧光蛋白（GFP）基因结合使用，以监测和优化表达和分泌。 灌注生物反应器将被设计为增强分泌蛋白的连续生产。 ***

9712916 Su This proposal seeks to improve the production of heterologous proteins in plant cell culture by integration of three new technologies: anew promoter; anew reporter gene; and an improved perfusion bioreactor. The recently identified strong, inducible, acid phosphatase promoter and signal sequence in Anchusa officinalis will be used in conjunction with the green fluorescent protein (GFP) gene as a reporter protein to monitor and optimize expression and secretion. A perfusion bioreactor will be designed to enhance continuous production of secreted proteins. ***