Integrated Bioprocessing/Molecular Strategies for Heterologous Protein Production in Perfusion Plant Cell Cultures

9712916这项建议旨在通过整合三项新技术来提高植物细胞培养中异源蛋白的产量：新的启动子；新的报告基因；以及改进的灌流生物反应器。新近发现的强、可诱导的酸性磷酸酶启动子和信号序列将与绿色荧光蛋白(GFP)基因一起作为报告蛋白用于监测和优化表达和分泌。将设计一种灌流生物反应器，以增强分泌蛋白质的持续生产。***

9712916 Su This proposal seeks to improve the production of heterologous proteins in plant cell culture by integration of three new technologies: anew promoter; anew reporter gene; and an improved perfusion bioreactor. The recently identified strong, inducible, acid phosphatase promoter and signal sequence in Anchusa officinalis will be used in conjunction with the green fluorescent protein (GFP) gene as a reporter protein to monitor and optimize expression and secretion. A perfusion bioreactor will be designed to enhance continuous production of secreted proteins. ***