US-Egypt Cooperative Research: An Anchored Comparative Map of the River Buffalo Genome

小行星9713618 描述：这个奖项是为了支持一个合作项目的詹姆斯E。Womack博士，德克萨斯州学院站的德克萨斯农工大学兽医病理学系，以及埃及开罗国家研究中心（NRC）细胞生物学系的Soheir El-Nahas博士。 这项联合研究的目的是利用埃及实验室产生的一组杂交体细胞，绘制最初用于美国实验室牛基因作图的比较锚基因座。 这些将包括比较性锚标记位点（CAT）引物（如适用）和定制设计的牛PCR引物，用于与CAT引物作图无关的锚基因座。 目标是建立一个河流布法罗基因组的比较图谱，以10厘摩的间隔锚定到牛和其他哺乳动物（包括人类和小鼠）的图谱上。 这些图谱将允许从其他物种的高分辨率图谱中“比较候选定位克隆”布法罗中具有经济价值的基因。 适用范围：这一研究项目将使两名经验丰富的研究人员能够在一个对美国和埃及的科学利益具有重大潜力的项目上进行合作。 该项目通过鉴定重要经济性状的基因和标记，对埃及水牛的改良具有巨大潜力。 美国实验室将能够继续寻求确定人类和牛之间的染色体同源性，因为大多数CAT引物尚未在牛中进行测试，因此未映射。 因此，埃及实验室发现的对布法罗有效的任何引物，在牛中也有很高的可能性有效，然后可以添加到牛图谱中。 合作筛选CAT引物的最终结果将是比任何一个实验室单独进行的筛选更有效。 该提案符合支持美国的INT目标- 在对双方都有利的领域开展对外合作。 该项目正得到美国-埃及联合基金方案的支助，该方案向两国的科学家和工程师提供赠款，以开展这些合作活动。 ***

9713618 Womack Description: This award is for support of a cooperative project by Dr. James E. Womack, Department of Veterinary Pathobiology, Texas A&M University in College Station, TX, and Dr. Soheir El-Nahas, Department of Cell Biology at the National Research Center (NRC) in Cairo, Egypt. The objective of this joint research is to utilize a panel of hybrid somatic cells, generated in the Egyptian laboratory, to map comparative anchor loci developed initially for use in the cattle gene mapping in the US laboratory. These will include comparative anchor tagged sites (CATs) primers where applicable, and custom designed bovine PCR primers for anchor loci recalcitrant to mapping with CATs primers. The goal is to build a comparative map of river buffalo genome, anchored at ten centimorgan intervals to the maps of cattle and other mammals including human and mice. These maps will permit "comparative candidate positional cloning" of genes of economic interest in buffalo from the high resolution maps of other species. Scope: This research project will allow two experienced researchers to collaborate on a project of significant potential to the scientific interests in the United States and in Egypt. The project has great potential for the improvement of buffaloes in Egypt through the identification of genes for economically important traits and markers. The US laboratory will be able to continue its pursuit of identifying the chromosomal homology between humans and cattle, since most of the CATs primers remain untested in cattle and consequently are unmapped. Thus any primers discovered to work in buffalo by the Egyptian laboratory will have a high probability of working in cattle and can then be added to the cattle map. The net result of the collaboration to screen the CATs primers will be a more efficient screen than either laboratory can perform alone. This proposal meets the INT objective of supporting U.S.- foreign collaboration in areas that have shared benefits for both sides. This project is being supported under the US- Egypt Joint Fund Program, which provides grants to scientists and engineers in both countries to carry out these cooperative activities. ***